#43279 Arginase-1 (D4E3M™) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
|Arginase-1 (D4E3M™) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total arginase-1 protein. This antibody does not cross-react with arginase-2.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein.|
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Confocal immunofluorescent analysis of mouse liver (positive; left) or small intestine (negative; right) using Arginase-1 (D4E3M™) Rabbit mAb (Alexa Fluor® 647 Conjugate) (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) using Arginase-1 (D4E3M™) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (red). BMDMs were differentiated with M-CSF (20 ng/ml, 7 d) and activated with either IL-4/cAMP (20 ng/ml, 0.5 mM, 24 hr; left, positive) or LPS/IFNγ (50 ng/ml, 20 ng/ml, 24 hr; right, negative). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087.
L-arginine plays a critical role in regulating the immune system (1-3). In inflammation, cancer and certain other pathological conditions, myeloid cell differentiation is inhibited leading to a heterogeneous population of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs). MDSCs are recruited to sites of cancer-associated inflammation and express high levels of arginase-1 (4). Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine and urea (5). Thus MDSCs increase the catabolism of L-arginine resulting in L-arginine depletion in the inflammatory microenvironment of cancer (4, 6). The reduced availability of L-arginine suppresses T-cell proliferation and function and thus contributes to tumor progression (4, 6). Arginase-1 is of great interest to researchers looking for a therapeutic target to inhibit the function of MDSCs in the context of cancer immunotherapy (7). In addition, research studies have demonstrated that Arginase-1 distinguishes primary hepatocellular carcinoma (HCC) from metastatic tumors in the liver, indicating its value as a potential biomarker in the diagnosis of HCC (8, 9).
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- Raber, P. et al. (2012) Immunol Invest 41, 614-34.
- Wesolowski, R. et al. (2013) J Immunother Cancer 1, 10.
- Sang, W. et al. (2015) Tumour Biol 36, 3881-6.
- Geramizadeh, B. and Seirfar, N. (2015) Hepat Mon 15, e30336.
|93668 Arginase-1 (D4E3M™) XP® Rabbit mAb|
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
D4E3M is a trademark of Cell Signaling Technology. Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.