CST logo

#44463 Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb

 

Shopping Days対象製品 対象製品の中から、まとめて購入で一部製品が無料に!
*ウェブで専用注文書を作成し、代理店へご注文ください。

をクリックすると選べます。

CSTコード 包装
希望納入価格 (円)
Shopping Daysで購入
国内在庫 i
2019年2月22日15時50分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#44463S100 μL66,000
ログインすると国内在庫状況がご確認いただけます。
感度分子量 (kDa)抗体の由来貯法
トランスフェクト110-130, 125-140, 180-200Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト マウス、ラット
44463 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb detects immunoprecipitated or transfected levels of Axl/Mer/Tyro3 protein phosphorylated at Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681). The antibody may cross-react with other phosphorylated tyrosine proteins.
使用抗原
Monoclonal antibody is produced by immunizing animals with synthetic phospho-peptides corresponding to residues surrounding Tyr698/Tyr749 of human Axl/Mer protein.

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて44463 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H1299 and SK-MEL-5 cells, untreated (-) or treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min, using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb (upper) and Axl (C89E7) Rabbit mAb #8661 (lower 1), Mer (D21F11) XP® Rabbit mAb #4319 (lower 2), and Tyro3 (D38C6) Rabbit mAb #5585 (lower 3).

IP

IP

Immunoprecipitation of Phospho-Axl (Tyr698) protein from NCI-H1299 cells treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Axl (C89E7) Rabbit mAb #8661. Western blot analysis was performed using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb (upper) and Axl (C89E7) Rabbit mAb #8661 (lower). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

IP

IP

Immunoprecipitation of Phospho-Mer (Tyr749) protein from SK-MEL-5 cells treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Mer(D21F11) XP® Rabbit mAb #4319. Western blot analysis was performed using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb (upper) and Mer (D21F11) XP® Rabbit mAb #4319 (lower). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.


IP

IP

Immunoprecipitation of Phospho-Tyro3 (Tyr681) protein from SK-MEL-5 cells treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Tyro 3 (D38C6) Rabbit mAb #5585. Western blot analysis was performed using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb (upper) and Tyro3 (D38C6) Rabbit mAb #5585 (lower). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

バックグラウンド

Axl, Mer and Tyro3 are three members of the TAM family receptor tyrosine kinase that share a common NCAM (neural adhesion molecule)-related extracellular domain and a conserved intracellular tyrosine kinase domain. These receptors bind common homologous vitamin K dependent protein GAS6 and protein S to activate down stream signaling pathway (1). TAM family receptors is involved in the development of immune, nervous, vascular and reproductive systems, autoimmune disease, cancer drug resistance and tumor immunity response (2-5). Axl Tyr698, Mer Tyr749 and Tyro3 Tyr681 are conserved autophosphorylation sites located at the activation loop of the tyrosine kinase domain. Phosphorylation at these sites is required for full kinase activation of each of the corresponding receptors (6,7).

使用例
 
関連製品
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
4319   Mer (D21F11) XP® Rabbit mAb
5585   Tyro3 (D38C6) Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7074   Anti-rabbit IgG, HRP-linked Antibody
8661   Axl (C89E7) Rabbit mAb

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications