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#45170 Phospho-LAT (Tyr255) Antibody

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2019年3月22日15時35分 現在
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#45170S100 μL66,000

LAT 製品一覧

感度分子量 (kDa)抗体の由来貯法
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最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


Phospho-LAT (Tyr255) Antibody recognizes endogenous levels of LAT protein only when phosphorylated at Tyr255.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr255 of human LAT protein, isoform 1. Antibodies are purified by protein A and peptide affinity chromatography.

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※下記の社内データは、すべて45170 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved Jurkat and EL4 cells, treated with isotype control (-) or treated with cross-linked anti-CD3 plus anti-CD28 (+; 10 ug/mL each, 5 min), using Phospho-LAT (Tyr255) Antibody (upper) and LAT Antibody #9166 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved Jurkat cells, treated with isotype control (-) or with cross-linked anti-CD3 plus anti-CD28 (+; 10 ug/mL each, 5 min), using Phospho-LAT (Tyr255) Antibody. The phospho-specificity of the antibody was verified by pre-incubating the antibody with no peptide (left), LAT (Tyr255) phosphopeptide (center), and LAT (Tyr255) non-phosphopeptide (right).



Immunoprecipitation of phospho-LAT (Tyr255) from Jurkat cell extracts that were serum-starved and cross-linked with anti-CD3 plus anti-CD28 (10 ug/mL each, 5 min). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Phospho-LAT (Tyr255) Antibody. Western blot analysis was performed using Phospho-LAT (Tyr255) Antibody. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.


LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).

Research studies have shown that LAT is also phosphorylated at Tyr225 by ZAP-70 and Syk following activation of the TCR, which facilitates its binding to Grb2 and Vav and subsequent downstream activation of ERK (5-7).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
25361   SLP-76 (E4N7E) Rabbit mAb
2729   Normal Rabbit IgG
3581   Phospho-LAT (Tyr171) Antibody
3584   Phospho-LAT (Tyr191) Antibody
5127   Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
73778   Protein A Magnetic Beads
7727   Biotinylated Protein Ladder Detection Pack
9166   LAT Antibody
92711   Phospho-SLP-76 (Ser376) (D7S1K) XP® Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

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Phospho-LAT (Tyr255) Antibody

Immune Cell Signaling Pathways