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#4638 TrkA and TrkB Antibody Sampler Kit

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2019年1月17日15時10分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619 20 µl WB, IP H, R M 140 Rabbit IgG
Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 20 µl WB, IP H, R M 140 Rabbit IgG
TrkA (12G8) Rabbit mAb #2510 20 µl WB, IHC-P H 140 Rabbit IgG
TrkB (80E3) Rabbit mAb #4603 20 µl WB H, M, R 90, 140 Rabbit IgG
Trk (pan) (A7H6R) Rabbit mAb #92991 20 µl WB, IP H, M, R 120-140 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, R=Rat, M=Mouse

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells stably transfected with either TrkA or TrkB, left untreated or treated with NGF or BDNF, respectively, using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb (upper) and pooled TrkA/TrkB antibodies (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells stably transfected with TrkA or TrkB, and treated with NGF or BDNF, respectively, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb (upper) and pooled TrkA/TrkB Antibodies (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal mouse brain, rat brain and NIH/3T3/TrkB cells using TrkB (80E3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, NIH/3T3-TrkA, and NIH/3T3-TrkB using TrkA (12G8) Rabbit mAb (upper) TrkB (80E3) Rabbit mAb Antibody #4603 (middle) and PLCγ1 (D9H10) XP® Rabbit mAb #5690 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse neonatal and rat fetal brain using Trk (pan) (A7H6R) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or NGF-treated 3T3/TrkA and PC12 cells using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or NGF-treated PC12 cells using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3-TrkA (left), NIH/3T3-TrkB (middle) and NIH/3T3-TrkC (right) cell pellets using TrkA (12G8) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from M0-91, KM12 cells and mouse neonatal brain using Trk (pan) (A7H6R) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TrkA (12G8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IP

IP

Immunoprecipitation of mouse neonatal brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Trk (pan) (A7H6R) Rabbit mAb. Western blot analysis was performed using Trk (pan) (A7H6R) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using TrkA (12G8) Rabbit mAb.

バックグラウンド

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10).

The phosphorylation sites are conserved between TrkA and TrkB: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674/675 of TrkA to Tyr706/707 in TrkB of the human sequence (14). TrkB is overexpressed in tumors, such as neuroblastoma, prostate adenocarcinoma, and pancreatic ductal adenocarcinoma (15). Research studies have shown that in neuroblastomas, overexpression of TrkB correlates with an unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF) (16-18). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms’ tumors and this isoform may act as a dominant-negative regulator of TrkB signaling (17).

使用例
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関連製品
1435   TrkA Blocking Peptide
2505   TrkA Antibody
3376   TrkC (C44H5) Rabbit mAb
3897   Human Brain-Derived Neurotrophic Factor (BDNF)
4606   TrkB Antibody
4607   TrkB (80G2) Rabbit mAb
7075   Anti-biotin, HRP-linked Antibody
9141   Phospho-TrkA (Tyr490) Antibody

Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

TrkA and TrkB Antibody Sampler Kit

Immune Cell Signaling Pathways

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