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#4670 Loading Control Antibody Sampler Kit (HRP Conjugate)

 
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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#4670T1 Kit84,000
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4670 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
COX IV (3E11) Rabbit mAb (HRP Conjugate) #5247 20 µl WB H, R, Mk, Z, B, Pg 17 Rabbit IgG
β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) #5346 20 µl WB H, M, R, Mk, Z, B C 55 Rabbit IgG
GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate) #8884 20 µl WB H, M, R, Mk Pg 37 Rabbit IgG
β-Actin (D6A8) Rabbit mAb (HRP Conjugate) #12620 20 µl WB H, M, R, Mk, Dm, Z Hm, C, B, Pg 45 Rabbit IgG
Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate) #12648 20 µl WB H, M, R, Mk Hm, C, Dm, X, Z, B 17 Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting
Reactivity Key: H=Human, R=Rat, Mk=Monkey, Z=Zebrafish, B=Bovine, Pg=Pig, M=Mouse, Dm=D. melanogaster

用途 (希釈倍率)
ウェスタンブロッティング (1:1,000)
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using β-Tubulin (9F3) Rabbit mAb (HRP Conjugate).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, HeLa and COS cell lines, using COX IV (3E11) Rabbit mAb (HRP Conjugate).

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and HeLa cells using GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb (HRP Conjugate).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate).

バックグラウンド

Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, COX IV, GAPDH, histone H3 and the α- and β-tubulins as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Recent work has demonstrated that GAPDH plays roles in apoptosis (2), gene expression (3), and nuclear transport (4). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (5). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (6). Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (7-9). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of protons across the mitochondrial inner membrane to drive ATP synthesis (10).

使用例
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関連製品
3683   GAPDH (14C10) Rabbit mAb (HRP Conjugate)
5125   β-Actin (13E5) Rabbit mAb (HRP Conjugate)
5192   Histone H3 (3H1) Rabbit mAb (HRP Conjugate)
5247   COX IV (3E11) Rabbit mAb (HRP Conjugate)
5346   β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)
9099   α-Tubulin (11H10) Rabbit mAb (HRP Conjugate)

XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Loading Control Antibody Sampler Kit (HRP Conjugate)

Metabolic Reprogramming in Disease

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