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#4749 Integrin Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
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2019年3月20日11時40分 現在
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#4749T1 Kit99,000
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4749 の推奨プロトコール i

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Integrin α5 Antibody #4705 20 µl WB H, M, Mk 150 Rabbit
Integrin αV Antibody #4711 20 µl WB, IP H, R 135, 140 Rabbit
Integrin β4 (D8P6C) XP® Rabbit mAb #14803 20 µl WB, IHC-P, F H 210 Rabbit IgG
Integrin α4 (D2E1) XP® Rabbit mAb #8440 20 µl WB, IP, IF-IC, F H, M, R 70, 140, 150, Rabbit IgG
Integrin β1 (D2E5) Rabbit mAb #9699 20 µl WB, IHC-P H 115, 135 Rabbit IgG
Integrin β3 (D7X3P) XP® Rabbit mAb #13166 20 µl WB, IHC-P H, M 100 Rabbit IgG
Integrin β5 (D24A5) Rabbit mAb #3629 20 µl WB, IP, IF-IC H, M, R, Mk 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat

社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549, NIH/3T3 and COS cell lines using Integrin α5 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 and C6 cells using Integrin αV Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Integrin β5 (D24A5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Integrin β1 (D2E5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Integrin α4 (D2E1) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from U-87 MG, HUVEC and C2C12 cell lines using Integrin β3 (D7X3P) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, LNCaP, and A-204 cells using Integrin β4 (D8P6C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells using Integrin β5 (D24A5) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β1 (D2E5) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Integrin α4 (D2E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat (positive; left) and K-562 (negative; right) cells using Integrin α4 (D2E1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Integrin β1 (D2E5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 (left) and LNCaP (right) cell pellets using Integrin β4 (D8P6C) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skin using Integrin β1 (D2E5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human testes using Integrin β3 (D7X3P) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A-204 cells (blue) and A-431 cells (green) using Integrin β4 (D8P6C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


バックグラウンド

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

使用例
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Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Integrin Antibody Sampler Kit

Metabolic Reprogramming in Disease

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