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#4750 PAK 1/2/3 Antibody Sampler Kit

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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606 20 µl WB H, M, GP R 61 to 67 (PAK2), 68 to 74 (PAK1/3) Rabbit
Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605 20 µl WB H, M, R, GP 61 to 67 (PAK2), 68 to 74 (PAK1/3) Rabbit
Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601 20 µl WB H, M, GP R 61 to 67 (PAK2), 68 to 74 (PAK1/3) Rabbit
Phospho-PAK2 (Ser20) Antibody #2607 20 µl WB H, M, GP R 61 to 67 Rabbit
PAK1 Antibody #2602 20 µl WB, IP H, M, R, Mk, GP 68 Rabbit
PAK2 (C17A10) Rabbit mAb #2615 20 µl WB H, M, Mk 61 Rabbit IgG
PAK3 Antibody #2609 20 µl WB, IP H, M, R 65 Rabbit
PAK1/2/3 Antibody #2604 20 µl WB H, M, R, Mk, GP 61 (PAK2), 68 (PAK1/3) Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, GP=Guinea Pig, R=Rat, Mk=Monkey

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1/2/3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP (1 µM ) for the indicated times, using Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody.


Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from guinea pig neutrophils stimulated with 1 µM fMLP for indicated times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.)

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells cotransfected with activated cdc42 and GST-PAK2 (lane 1), transfected with GST-PAK2 alone (lane 2) or activated cdc42 alone (lane 3), using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody (upper) or PAK1/2/3 Antibody #2604 (lower). This antibody detects GST-PAK2 signal only when it is cotransfected with activated cdc42, indicating that this antibody is phospho-specific.


Western Blotting

Western Blotting

Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for the indicated times, using Phospho-PAK2 (Ser20) Antibody (upper) or PAK1/2/3 Antibody #2604 (lower).

Western Blotting

Western Blotting

Western blot analysis of mouse brain extract and recombinant GST-PAK3, using PAK3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, C2C12 and COS cells using PAK2 (C17A10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated with 0.4 M sorbitol for indicted times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (upper) or PAK2 antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for indicated times, using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody (upper) and PAK1/2/3 Antibody #2604 (lower).

IP

IP

Immunoprecipitation of PAK3 from mouse brain extract, using PAK3 Antibody, followed by Western blot analysis using PAK3 Antibody (left), PAK1 Antibody #2602 (middle) or PAK2 Antibody #2608 (right).


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or PAK1 siRNA, using PAK1 Antibody #2602 and p42 MAP Kinase (Erk2) Antibody #9108. The PAK1 Antibody confirms silencing of PAK1 expression, and p42 MAP Kinase (Erk2) Antibody is used to control for loading and specificity of PAK1 siRNA.

IP

IP

Immunoprecipitation of PAK1 from C6 and SH-SY5Y cells followed by Western blot analysis, using PAK1 Antibody.

バックグラウンド

The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including serines 199 and 204 of PAK1 and serines 192 and 197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation of Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8).

使用例
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6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
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9999   Nonfat Dry Milk

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

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