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#4763 Translation Initiation Complex Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#4763T1 Kit115,000
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
eIF4A (C32B4) Rabbit mAb #2013 20 µl WB H, M, R, Mk 48 Rabbit IgG
eIF4A1 Antibody #2490 20 µl WB H, M, R, Mk 48 Rabbit
Phospho-eIF4B (Ser422) Antibody #3591 20 µl WB H, M, R, Mk 80 Rabbit
eIF4B Antibody #3592 20 µl WB H, M, R, Mk 80 Rabbit
Phospho-eIF4E (Ser209) Antibody #9741 20 µl WB H, M, R, Mk 25 Rabbit
eIF4E (C46H6) Rabbit mAb #2067 20 µl WB, IP, IHC-P H, M, R, Mk 25 Rabbit IgG
Phospho-eIF4G (Ser1108) Antibody #2441 20 µl WB, IP, IF-IC H, M, R, Hm, Mk, B 220 Rabbit
eIF4G (C45A4) Rabbit mAb #2469 20 µl WB, IHC-P, IF-IC, F H, M, R, Mk 220 Rabbit
eIF4H (D85F2) XP® Rabbit mAb #3469 20 µl WB, IP, IF-IC H, M, R, Mk 25, 27 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster, B=Bovine

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody (upper) or eIF4E Antibody #9742 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells expressing GST-eIF4GI Ser1192Ala or GST-eIF4GI Ser1108Ala mutant protein, using Phospho-eIF4G (Ser1108) Antibody. (Provided by Brian Raught, McGill University, Montreal, QuŽbec.)

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, 293 and NIH/3T3 cells using eIF4B Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following siRNA transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using eIF4A1 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using eIF4G (C45A4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using eIF4A (C32B4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2, HeLa and NBT-II cells using eIF4H (D85F2) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human glioblastoma using eIF4G (C45A4) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells (left) and MCF-7 cells (right) using eIF4H (D85F2) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® eIF4E siRNA I #6311 or SignalSilence® eIF4E siRNA II #6554 (+), using eIF4E (C46H6) Rabbit mAb #2067 and α-Tubulin (11H10) Rabbit mAb #2125. The eIF4E (C46H6) Rabbit mAb confirms silencing of eIF4E expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of eIF4E siRNA.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells either rapamycin-treated (left) or serum-treated (right), using Phospho-eIF4G (Ser1108) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF4G (C45A4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF4E (C46H6) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using eIF4G (C45A4) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4E (C46H6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using eIF4G (C45A4) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7).

使用文献
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2013   eIF4A (C32B4) Rabbit mAb
2067   eIF4E (C46H6) Rabbit mAb
2425   eIF4A (F52) Antibody
2441   Phospho-eIF4G (Ser1108) Antibody
2444   eIF4H Antibody
2469   eIF4G (C45A4) Rabbit mAb
2490   eIF4A1 Antibody
2498   eIF4G Antibody
2617   eIF4G (C65H5) Rabbit mAb
2858   eIF4GI Antibody
3591   Phospho-eIF4B (Ser422) Antibody
3592   eIF4B Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9741   Phospho-eIF4E (Ser209) Antibody
9742   eIF4E Antibody

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Translation Initiation Complex Antibody Sampler Kit

Immune Cell Signaling Pathways

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