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#47767 Tricarboxylic Acid Cycle Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Fumarase (D9C5) Rabbit mAb #4567 20 µl WB H, M, R, Hm, Mk 49 Rabbit IgG
IDH2 (D8E3B) Rabbit mAb #56439 20 µl WB, IP H, M, R 43 Rabbit IgG
IDH1 (D2H1) Rabbit mAb #8137 20 µl WB, IP, IF-IC, F H, M, R, Mk 46 Rabbit IgG
Citrate Synthase (D7V8B) Rabbit mAb #14309 20 µl WB, IF-IC H, M, R, Hm, Mk 45 Rabbit IgG
ACO2 (D6D9) XP® Rabbit mAb #6571 20 µl WB, IP, IF-IC H, M, R, Hm, Mk 85 Rabbit IgG
MPC2 (D4I7G) Rabbit mAb #46141 20 µl WB, IP H, M, R, Mk 14 Rabbit IgG
MPC1 (D2L9I) Rabbit mAb #14462 20 µl WB, IP H, M, R, Mk 12 Rabbit IgG
DLST (D22B1) XP® Rabbit mAb #11954 20 µl WB, IP, IF-IC H 50 Rabbit IgG
SDHA (D6J9M) XP® Rabbit mAb  #11998 20 µl WB, IP, IHC-P, IF-IC H, M, R, Hm, Mk 70 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IDH1 (D2H1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untransfected (-) or transfected with a Myc-tagged cDNA expression construct encoding human fumarase (hFumarase-Myc; +), using Fumarase (D9C5) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ACO2 (D6D9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DLST (D22B1) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MPC1 (D2L9I) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Citrate Synthase (D7V8B) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MPC2 (D4I7G) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and ACHN cells using IDH2 (D8E3B) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using IDH1 (D2H1) Rabbit mAb (blue) compared to concentration-matched Rabbit

(DA1E) mAb IgG XP® Isotype Control #3900 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Fumarase (D9C5) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using ACO2 (D6D9) XP® Rabbit mAb (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of DLST from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DLST (D22B1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DLST (D22B1) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

IP

IP

Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Citrate Synthase (D7V8B) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human MPC1 protein (hMPC1-DDK; +) or HA-tagged full-length human MPC2 protein (hMPC2-HA; +), using MPC1 (D2L9I) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (upper middle), HA-Tag (C29F4) Rabbit mAb #3724 (lower middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Both expression vectors were provided by Dr. Jared Rutter's laboratory at the University of Utah School of Medicine.

IP

IP

Immunoprecipitation of IDH2 from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IDH2 (D8E3B) Rabbit mAb. Western blot analysis was performed using IDH2 (D8E3B) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T cells using IDH1 (D2H1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using DLST (D22B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IP

IP

Immunoprecipitation of MPC1 from Huh7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MPC1 (D2L9I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MPC1 (D2L9I) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The tricarboxylic acid (TCA) cycle includes various enzymatic reactions that constitute a key part of cellular aerobic respiration. The transport of the glycolytic end product pyruvate into mitochondria and the decarboxylation of pyruvate in the TCA cycle generate energy through oxidative phosphorylation under aerobic conditions (1,2). Two inner mitochondrial membrane proteins, mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2), form a 150 kDa complex and are essential proteins in the facilitated transport of pyruvate into mitochondria (1,2). Citrate synthase catalyzes the first and rate-limiting reaction of the TCA cycle (3). Mitochondrial aconitase 2 (ACO2) catalyzes the conversion of citrate to isocitrate via cis-aconitate (4). IDH1 and IDH2 are two of the three isocitrate dehydrogenases that catalyze oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG) (5). IDH1 functions as a tumor suppressor in the cytoplasm and peroxisomes, whereas IDH2 is in mitochondria and is involved in the TCA cycle (5). Mutations in IDH2 have also been identified in malignant gliomas (6). Dihydrolipoamide succinyltransferase (DLST) is a subunit of the α-ketoglutarate dehydrogenase complex, a key enzymatic complex in the TCA cycle (7). Succinate dehydrogenase subunit A (SDHA) is a component of the TCA cycle and the electron transport chain and is involved in the oxidation of succinate (8). Fumarase catalyzes the conversion of fumarate to malate (9). Fumarase deficiency leads to the accumulation of fumarate, an oncometabolite that has been shown to promote epithelial-to-mesenchymal-transition (EMT), a developmental process that has been implicated in oncogenesis (10).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
11954   DLST (D22B1) XP® Rabbit mAb
11998   SDHA (D6J9M) XP® Rabbit mAb 
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
14309   Citrate Synthase (D7V8B) Rabbit mAb
14462   MPC1 (D2L9I) Rabbit mAb
4567   Fumarase (D9C5) Rabbit mAb
46141   MPC2 (D4I7G) Rabbit mAb
56439   IDH2 (D8E3B) Rabbit mAb
6571   ACO2 (D6D9) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8137   IDH1 (D2H1) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
SignalFire is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

本製品は試験研究用です。

Tricarboxylic Acid Cycle Antibody Sampler Kit

Metabolic Reprogramming in Disease

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