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#48243 Cardiogenesis Marker Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#48243T1 Kit99,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
NKX2.5 (E1Y8H) Rabbit mAb #8792 20 µl WB, IF-IC, F H 30-42 Rabbit IgG
GATA-6 (D61E4) XP® Rabbit mAb #5851 20 µl WB, IF-IC, F, ChIP, ChIP-seq H M, R, Dg, Pg 55 Rabbit IgG
MEF2C (D80C1) XP® Rabbit mAb #5030 20 µl WB, IP, IF-IC H, M 50-60 Rabbit IgG
α-Actinin (D6F6) XP® Rabbit mAb #6487 20 µl WB, IF-IC H, M, R, Mk Z 100 Rabbit IgG
Troponin I (D6F8) Rabbit mAb #13083 20 µl WB H, M, R Mk 28 Rabbit IgG
Troponin T (Cardiac) Antibody #5593 20 µl WB H, R Mk 40 Rabbit
Connexin 43 Antibody #3512 20 µl WB, IHC-P, IHC-F, IF-F, IF-IC H, M, R, Mk, Z Dg, Pg 39, 41, 43, 44 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Z=Zebrafish

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated and PMA-treated C6, COS, HeLa and C2C12 cells using Connexin 43 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells lines using MEF2C (D80C1) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human cardiac Troponin T (isoform 1) construct (+), using Troponin T (Cardiac) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Huh7 and 293 cells using GATA-6 (D61E4) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NKX2.5 (E1Y8H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from human, mouse and rat heart tissue using Troponin I (D6F8) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HEK293 (positive, left) and PANC-1 (negative, right) cells, using NKX2.5 (E1Y8H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across FGFR2, a known target gene of GATA-6 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Connexin 43 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, undifferentiated (left) or differentiated for 3 days (right), using MEF2C (D80C1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).


Western Blotting

Western Blotting

Western blot analysis of extracts from human and rat heart and human skeletal muscle using Troponin T (Cardiac) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SNB19 cells using α-Actinin (D6F6) XP® Rabbit mAb (green) showing colocalization with actin filaments that were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and either GATA-6 (D61E4) XP® Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human FDPS Promoter Primers #13840, human FGFR2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SKOV3 cells (blue) and HUH-7 cells (green) using GATA-6 (D61E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of PANC-1 cells (blue) and HEK293 cells (green) using NKX2.5 (E1Y8H) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human heart, using Connexin 43 Antibody. (High magnification, inset).


IF-IC

IF-IC

Confocal immunofluorescent analysis of KM12 (left) and SK-OV-3 cells (right) using GATA-6 (D61E4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Connexin 43 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse heart using Connexin 43 Antibody in the presence of control peptide (left) or antigen specific peptide (lright).


IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen mouse heart tissue, using Connexin 43 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of confluent COS cells using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of rat optic nerve and ciliary epithelium using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

See notes for additional testing details.


バックグラウンド

Cardiogenesis is a complex developmental event involving numerous transcription factors. NKX2.5 is a member of the NKX homeobox transcription factor family, which plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Mutations in NKX2.5 are associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (1,2). GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (3-5). GATA-6 plays a critical role in endoderm development and knock out of GATA-6 is embryonic lethal due to defects in formation of the heart tube and a failure to develop extraembryonic endoderm (6). MEF2C is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (7,8). α-Actinin was first recognized as an actin cross-linking protein. The α-actinin protein interacts with a large number of proteins involved in signaling to the cytoskeleton, including those involved in cellular adhesion, migration, and immune cell targeting (9). The muscle isoforms 2 and 3 (ACTN2, ACTN3) localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (9). Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (10). Assays for measuring serum concentrations of cardiac muscle TnT (cTNT), as well as cTnI, have been reported for analyzing cardiac injury. Connexin 43 (Cx43) is a member of the large family of gap junction proteins, which assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (11,12).

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Illumina is a registered trademark of Illumina, Inc.
Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Cardiogenesis Marker Antibody Sampler Kit

Immune Cell Signaling Pathways

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