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#48488 DNMT3B (E4I4O) Rabbit mAb (Mouse Specific)


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2019年3月22日15時35分 現在
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#48488S100 μL56,000

DNMT3B 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性105Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


マウス -
48488 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫蛍光細胞染色 (IF-IC) (1:1600)フローサイトメトリー (1:800)クロマチン免疫沈降 (1:50)


DNMT3B (E4I4O) Rabbit mAb recognizes endogenous levels of total DNMT3B protein. This antibody does not cross-react with DNMT3A or DNMT1.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu561 of mouse DNMT3B protein.

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※下記の社内データは、すべて48488 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from mES, F9, and NIH/3T3 cell lines using DNMT3B (E4I4O) Rabbit mab (Mouse Specific) (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, NIH/3T3 cells show very low to no expression of DNMT3B.



Immunoprecipitation of DNMT3B from F9 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DNMT3B (E4I4O) Rabbit mAb (Mouse Specific). Western blot analysis was performed using DNMT3B (E4I4O) Rabbit mAb (Mouse Specific).



Confocal immunofluorescent analysis of mES (left, positive) and 3T3 (right, negative) cells using DNMT3B (E4I4O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells (blue) and mES cells (green) using DNMT3B (E4I4O) Rabbit mAb (Mouse Specific) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from mES cells and either DNMT3B (E4I4O) Rabbit mAb (Mouse Specific) or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Exo1 Exon 12 Primers #49170, mouse FH1 intron 9 primers, mouse RCC2 exon 3 primers and SimpleChIP® Mouse Oct-4 Promoter Primers #4653. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).

13326   DMAP1 Antibody
13451   DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific)
28692   5-Methylcytosine (5-mC) (D3S2Z) Rabbit mAb
32578   DNMT3A (D2H4B) Rabbit mAb
36836   5-Carboxylcytosine (5-caC) (D7S8U) Rabbit mAb
44145   DNMT3B (E9X7R) Rabbit mAb (Mouse Specific)
4653   SimpleChIP® Mouse Oct-4 Promoter Primers
49768   DNMT3A (E9P2F) Rabbit mAb
5032   DNMT1 (D63A6) XP® Rabbit mAb
51660   5-Hydroxymethylcytosine (5-hmC) (HMC31) Mouse mAb
56383   SimpleChIP® Plus Sonication Chromatin IP Kit
67259   DNMT3B (D7O7O) Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
74178   5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb
76853   SimpleDIP™ Methylated DNA IP (MeDIP) Kit
7727   Biotinylated Protein Ladder Detection Pack
88989   SimpleChIP® Universal qPCR Master Mix
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
95176   SimpleDIP™ Hydroxymethylated DNA IP (hMeDIP) Kit
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.


DNMT3B (E4I4O) Rabbit mAb (Mouse Specific)

Immune Cell Signaling Pathways