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#49561 Phospho-Tau (Thr205) (E7D3E) Rabbit mAb


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2019年3月25日15時35分 現在
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#49561S100 μL66,000

Tau 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性50-80Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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49561 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)免疫組織染色 (パラフィン) (1:600)免疫蛍光染色 (IF-F) (1:100)


IHC-P: 抗体希釈液 / 抗原賦活化


Phospho-Tau (Thr205) (E7D3E) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Thr205.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Thr205 of the human Tau protein.

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※下記の社内データは、すべて49561 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, untreated (-) or phosphatase-treated (+), and rat brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (upper) and Tau (D1M9X) Rabbit mAb #46687 (lower).



Immunoprecipitation of Phospho-Tau (Thr205) from mouse brain lysates. Lane 1 mouse brain lysate immunoprecipitation, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lanes 3, 4, and 5 represent three dilutions of the immunoprecipitation of mouse brain lysates at 1:50, 1:100, 1:200 respectively, and lane 6 is mouse brain lysate. Western blot analysis was performed using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's disease brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb in the presence of non-phospho-Tau (Thr205) peptide (left) or phospho-Tau (Thr205) peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse small intestine using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.



Confocal immunofluorescent analysis of mouse medulla oblangata using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (green). Blue = DAPI #4083 (fluorescent DNA dye).



Confocal immunofluorescent analysis of dentate gyrus in wild-type mouse brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (green). Antibody was pre-incubated with a non-phospho-Tau peptide (left), a phospho-Tau (Thr205) peptide (center), or without peptide (right) to confirm phospho-specificity. Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Phosphorylation of Tau at Thr205 is found in Alzheimer's disease brain. This phosphorylation is the result of the activity of glycogene synthetase-kinase 3 (GSK-3), protein-kinase A (PKA), and other kinases (4,5). Protein phosphatases have been shown to decrease the levels of phospho-Tau at Thr205, including protein phosphatase 5 (PP5) (6).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12885   Phospho-Tau (Thr181) (D9F4G) Rabbit mAb
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15013   Phospho-Tau (Ser416) (D7U2P) Rabbit mAb
15019   Animal-Free Blocking Solution (5X)
20194   Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred)
29957   Phospho-Tau (Ser199) Antibody
39357   Phospho-Tau (Ser202) (D4H7E) Rabbit mAb
4019   Tau (Tau46) Mouse mAb
46687   Tau (D1M9X) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
77348   Phospho-Tau (Ser214) (D1Q2X) Rabbit mAb
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
96628   Phospho-Tau Family Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-Tau (Thr205) (E7D3E) Rabbit mAb

Immune Cell Signaling Pathways