#49561 Phospho-Tau (Thr205) (E7D3E) Rabbit mAb
IHC-P: 抗体希釈液 / 抗原賦活化
|Phospho-Tau (Thr205) (E7D3E) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Thr205.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Thr205 of the human Tau protein.|
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Western blot analysis of extracts from mouse brain, untreated (-) or phosphatase-treated (+), and rat brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (upper) and Tau (D1M9X) Rabbit mAb #46687 (lower).
Immunoprecipitation of Phospho-Tau (Thr205) from mouse brain lysates. Lane 1 mouse brain lysate immunoprecipitation, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lanes 3, 4, and 5 represent three dilutions of the immunoprecipitation of mouse brain lysates at 1:50, 1:100, 1:200 respectively, and lane 6 is mouse brain lysate. Western blot analysis was performed using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human Alzheimer's disease brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb in the presence of non-phospho-Tau (Thr205) peptide (left) or phospho-Tau (Thr205) peptide (right).
Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.
Confocal immunofluorescent analysis of mouse medulla oblangata using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (green). Blue = DAPI #4083 (fluorescent DNA dye).
Confocal immunofluorescent analysis of dentate gyrus in wild-type mouse brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (green). Antibody was pre-incubated with a non-phospho-Tau peptide (left), a phospho-Tau (Thr205) peptide (center), or without peptide (right) to confirm phospho-specificity. Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
Phosphorylation of Tau at Thr205 is found in Alzheimer's disease brain. This phosphorylation is the result of the activity of glycogene synthetase-kinase 3 (GSK-3), protein-kinase A (PKA), and other kinases (4,5). Protein phosphatases have been shown to decrease the levels of phospho-Tau at Thr205, including protein phosphatase 5 (PP5) (6).
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