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#50722 H2A.Z (E9M5G) Rabbit mAb

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2019年3月25日15時35分 現在
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Histone H2A.Z 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性14Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

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50722 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)クロマチン免疫沈降 (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
H2A.Z (E9M5G) Rabbit mAb recognizes endogenous levels of total H2A.Z protein. This antibody does not cross-react with H2A protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human H2A.Z protein.

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社内データ

※下記の社内データは、すべて50722 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of HeLa, NIH/3T3, C6, and COS-7 cell lysates, in addition to 10 ng of recombinant H2A (rH2A) and H2A.Z (rH2A.Z) proteins, using Histone H2A.Z (E9M5G) Rabbit mAb (upper) and Histone H2A (D6O3A) Rabbit mAb #12349 (lower).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from A549 cells and either H2A.Z (E9M5G) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human SLC2A8 promoter primers, SimpleChIP® Human VAMP1 Promoter Primers #25921, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

Modulation of chromatin structure plays a critical role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z (2). Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability (2). Several homologous protein complexes, such as SWR-C (S. cerevisiae), TIP60 (D. melanogaster) and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome (3,4,5). This exchange of histone H2A variants changes histone-histone interactions in the nucleosome core and alters an acidic patch on the surface of the nucleosome, resulting in changes in nucleosome stability and binding of non-histone proteins such as HP1α (6,7).

使用例
 
関連製品
12349   Histone H2A (D6O3A) Rabbit mAb
2718   Histone H2A.Z Antibody
4486   SimpleChIP® Human α Satellite Repeat Primers
7003   20X LumiGLO® Reagent and 20X Peroxide
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
75336   Acetyl-Histone H2AZ (Lys4/Lys7) (D3V1I) Rabbit mAb
7631   Histone H2A.X (D17A3) XP® Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
96139   Acetyl-Histone H2AZ (Lys4/Lys7) (D3V1I) Rabbit mAb (Alexa Fluor® 488 Conjugate)
9933   Acetyl-Histone Antibody Sampler Kit
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

H2A.Z (E9M5G) Rabbit mAb

Metabolic Reprogramming in Disease

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