CST logo

#52420 Phospho-YAP/TAZ Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月20日11時40分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#52420T1 Kit99,000
ログインすると国内在庫状況がご確認いただけます。

YAP 製品一覧

52420 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-YAP (Ser109) Antibody #46931 20 µl WB, IP H, M, R, Mk 78 Rabbit
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008 20 µl WB, IP, IHC-P H, M, R 65-75 Rabbit IgG
Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb #13619 20 µl WB, IP H, M, R 75 Rabbit IgG
Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb #59971 20 µl WB, IP H, M, R 55 Rabbit IgG
YAP/TAZ (D24E4) Rabbit mAb #8418 20 µl WB, IP, IHC-P H, M, Mk 50, 70 Rabbit IgG
YAP (D8H1X) XP® Rabbit mAb #14074 20 µl WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq H, M, R, Hm, Mk B, GP, Hr 65-75 Rabbit IgG
TAZ (D3I6D) Rabbit mAb #70148 20 µl WB, IP, ChIP, ChIP-seq H, M 50 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using YAP/TAZ (D24E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). YAP protein isoform 1 Ser397 corresponds to Ser381 of YAP isoform 2, as reported by Zhao et al. (2010) Genes Dev 24, 72-85 (9).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using YAP (D8H1X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, RL-7 cells are negative for YAP protein expression.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-H2052 cells and YAP (D8H1X) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across CTGF, a known target gene of YAP (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cells using TAZ (D3I6D) Rabbit mAb (upper) and GAPDH (D16H11) XP® #5174 (lower). As expected, Raji cells are negative for TAZ.

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1 cells (human) and NIH/3T3 cells (mouse), untreated (-) or treated with λ phosphatase (+), using Phospho-YAP (Ser109) Antibody (upper) and YAP (D8H1X) XP® Rabbit mAb #14074 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1 and A-204 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+) using Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb (upper), TAZ (D3I6D) Rabbit MAb #70148 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Asterisk (*) indicates weak detection of phosphorylated YAP, due to sequence similarity in the regions surrounding TAZ (Ser89) and YAP (Ser127).


Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-H2052 cells and TAZ (D3I6D) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across SMYD3, a known target gene of TAZ (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using YAP/TAZ (D24E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of MDA-MB-231 cells, vehicle-treated (-) or treated with Forskolin #3828 (10 μM, 60 min; +) or epinephrine (10 μM, 60 min; +), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Note the induction of YAP (Ser127) phosphorylation after treatment with forskolin or epinephrine, consistent with the findings reported in Xu et al. (2012) [9].


Western Blotting

Western Blotting

Western blot analysis of extracts from MDA-MB-231 cells, vehicle treated (-) or treated with epinephrine (10 μM, 60 min; +) or Forskolin #3828 (10 μm, 60 min; +), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8547 (lower). YAP protein isoform 1 Ser397 corresponds to Ser381 of YAP isoform 2, as reported by Zhao, B. et al. (2010) Genes Dev 24, 72-85 (9).

IP

IP

Immunoprecipitation of YAP protein from A-204 cell extracts using Rabbit (DA1E) mAb XP® Isotype Control #3900 (lane 2) or YAP (D8H1X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using YAP (D8H1X) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-2052 cells and either YAP (D8H1X) XP® Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CTGF Promoter Primers #14927, human SMYD3 intron 2 primers, and SimpleChIP® Human CTGF Upstream Primers #14928. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCI-H2052 cells and either TAZ (D3I6D) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CTGF Promoter Primers #14927, human SMYD3 intron 2 primers, and SimpleChIP® Human CTGF Upstream Primers #14928. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using using Phospho-YAP (Ser109) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). RL-7 cells are negative for YAP expression, confirming specificity of the antibody.

IP

IP

Immunoprecipitation of Phospho-TAZ (Ser89) from A-204 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb. Western blot analysis was performed using Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody. Asterisk (*) indicates weak detection of phosphorylated YAP, due to sequence similarity in the regions surrouding TAZ (Ser89) and YAP (Ser127).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using YAP/TAZ (D24E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb.

IP

IP

Immunoprecipitation of Phospho-YAP (Ser109) from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Phospho-YAP (Ser109) Antibody. Western blot analysis was performed using Phospho-YAP (Ser109) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded COS-7 cell pellets, transfected with YAP (left) or TAZ (right), using YAP/TAZ (D24E4) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma, control (left) or λ-phosphatase treated (right), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb.

Isoform Specificity

Isoform Specificity

Schematic diagram showing annotation of the amino acid sequence surrounding Ser397 in different human YAP isoforms. Asterisk (*) indicates annotation of the phosphorylation site as described in Zhao et al. 2010 [9].

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, A-204 (left) and RL-7 (right), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using YAP (D8H1X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded PANC-1 (left) and RL-7 (right) cell pellets using YAP (D8H1X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human benign prostatic hyperplasia using YAP (D8H1X) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of RL-7 cells (blue) and A-204 cells (green) using YAP (D8H1X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. 


IF-IC

IF-IC

Confocal immunofluorescent analysis of low confluence MCF 10A cells (left), high confluence MCF 10A (center), and YAP negative RL-7 cells (right) using YAP (D8H1X) XP® Rabbit mAb (green). Blue pseudocolor in lower images = DRAQ5® #4084 (fluorescent DNA dye). Increased nuclear localization of YAP protein is seen in low confluence (proliferating) cells.

バックグラウンド

YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. YAP and TAZ are structurally and functionally similar, but exhibit differential patterns of expression among cells and tissues that suggest partially non-redundant functions (1). YAP and TAZ are dynamically regulated in response to internal and external cellular signals. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with transcription factors (e.g., TEA domain proteins) that regulate the transcription of genes that control proliferation and cell survival (2). The subcellular localization of YAP and TAZ is dynamically regulated by a kinase cascade that regulates the phosphorylation status of key residues within YAP and TAZ. Phosphorylation of YAP and TAZ (e.g., Ser109, Ser127, Ser397 in YAP; Ser89 in TAZ) results in their cytoplasmic translocation, sequestration by 14-3-3 proteins, and recruitment of the β-TrCP (SCF) ubiquitin ligase complex (3,4). This complex ubiquitinates YAP and TAZ, triggering their proteolytic degradation in the proteasome, thereby altering the transcription of genes that control proliferation and cell survival (3-5).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Illumina is a registered trademark of Illumina, Inc.
Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Phospho-YAP/TAZ Antibody Sampler Kit

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications