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#52496 HIF-1α (D1S7W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)

 

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HIF1A 製品一覧

感度抗体の由来貯法
内在性Rabbit IgG4℃
種交差性 (社内試験済)
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社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

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52496 の推奨プロトコール i

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特異性・感度
HIF-1α (D1S7W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total HIF-1α protein. This antibody does not cross-react with HIF-2α protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu478 of human HIF-1α protein.

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社内データ

※下記の社内データは、すべて52496 の推奨プロトコールで実験した結果です。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of U-2 OS cells, untreated (blue) or treated with DMOG (1 mM, 6 hr; green), using HIF-1α (D1S7W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (dashed lines).

バックグラウンド

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VHL) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function (10).

使用例
 
関連製品
36169   HIF-1α (D1S7W) XP® Rabbit mAb

XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

本製品は試験研究用です。

HIF-1α (D1S7W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)

Immune Cell Signaling Pathways

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