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#52928 Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit mAb

 

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2019年1月17日15時10分 現在
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FGFR1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性120, 145Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト マウス、ラット
52928 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:100)免疫蛍光細胞染色 (IF-IC) (1:800)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit mAb recognizes endogenous levels of FGFR1 protein when phosphorylated at Tyr653 and/or Tyr654. This antibody exhibits some cross-reactivity with FGF Receptors 2 and 4 when overexpressed exogenously. Based on sequence comparisons, cross-reactivity with FGF Receptor 3 is possible but has not been experimentally confirmed.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr653 of human FGFR1 protein.

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社内データ

※下記の社内データは、すべて52928 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-204 cells, untreated (-) or treated with heparin (1 μg/mL, 5 min) followed by the Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #61977 (100 ng/mL, 5 min, +), using Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit mAb (upper) and FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740 (lower).

IP

IP

Immunoprecipitation of Phospho-FGF receptor 1 (Tyr653/654) protein from A-204 cell treated with heparin (1 μg/mL, 5 min) followed by the Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #61977 (100 ng/mL, 5 min). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-FGF Receptor 1(Tyr653/654) (D4X3D) Rabbit mAb. Western blot analysis was performed using Phospho-FGF Receptor 1(Tyr653/654) (D4X3D) Rabbit mAb as primary antibody. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of serum-starved A-204 cells, untreated (left), treated with heparin (1 μg/mL, 5 min) followed by the addition of Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #61977 (100 ng/mL, 5 min; center), or treated with heparin/FGF2 and post-processed with λ-phosphatase (2 hr; right), using Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit mAb (upper, green) and FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740 (lower, green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


バックグラウンド

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

使用例
 
関連製品
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
3471   Phospho-FGF Receptor (Tyr653/654) Antibody
3472   FGF Receptor 1 Antibody
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
7003   20X LumiGLO® Reagent and 20X Peroxide
7074   Anti-rabbit IgG, HRP-linked Antibody
9740   FGF Receptor 1 (D8E4) XP® Rabbit mAb

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit mAb

Immune Cell Signaling Pathways

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