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#53641 MLL3 (D1S1V) Rabbit mAb

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2019年3月22日15時35分 現在
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#53641S100 μL56,000
感度分子量 (kDa)抗体の由来貯法
内在性540Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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53641 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのMLL3 タンパク質を検出します。
ヒトのMLL3 タンパク質のAla2110 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて53641 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, HCT 116, and K-562 cells using MLL3 (D1S1V) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, HCT 116 cells are negative for MLL3 expression.


The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6).

MLL3, also known as histone-lysine N-methyltransferase 2C (KMT2C), is a large 540 kDa protein that functions as part of the MLL3/COMPASS-like complex to activate gene expression by mediating mono-methylation of histone H3 lysine 4 at gene enhancers (7). Enhancer-specific H3 lysine 4 mono-methylation (H3K4me1) correlates with increased levels of chromatin interactions between gene enhancers and promoters, while loss of this modification results in a reduction of enhancer-promoter interactions (8). Furthermore, H3K4me1 facilitates recruitment of the Cohesin complex, which may function to promote the interactions between gene enhancers and promoters (8). MLL3 is found to be mutated or have altered expression in a number of different cancers (9).

13073   WDR5 (D3X5B) Rabbit mAb
13105   WDR5 (D9E1I) Rabbit mAb
13171   RBBP5 (D3I6P) Rabbit mAb
14197   MLL1 (D6G8N) Rabbit mAb (Carboxy-terminal Antigen)
14689   MLL1 (D2M7U) Rabbit mAb (Amino-terminal Antigen)
44922   SET1B (D1U5D) Rabbit mAb
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
5019   ASH2L (D93F6) XP® Rabbit mAb
61702   SET1A (D3V9S) Rabbit mAb
63735   MLL2/KMT2B (D6X2E) Rabbit mAb (Carboxy-terminal Antigen)
6891   Menin (D45B1) XP® Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9723   Mono-Methyl-Histone H3 (Lys4) Antibody
9725   Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb
9751   Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


MLL3 (D1S1V) Rabbit mAb

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