#53749 Phospho-YAP (Ser109) (E5I9G) Rabbit mAb
|Phospho-YAP (Ser109) (E5I9G) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser109. Based on sequence similarity, the antibody may recognize TAZ protein when phosphorylated at Ser66.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser109 of human YAP protein.|
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Western blot analysis of extracts from PANC-1 cells and HeLa cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ-phosphatase (+), using Phospho-YAP (Ser109) (E5I9G) Rabbit mAb (upper), YAP (D8H1X) XP® Rabbit mAb #14074 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using using Phospho-YAP (Ser109) (E5I9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). RL-7 cells are negative for YAP expression, confirming specificity of the antibody. YAP expression is undetectable in RL-7 extracts, as predicted from published gene expression databases and as confirmed with other YAP antibodies.
Immunoprecipitation of Phospho-YAP (Ser109) protein from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-YAP (Ser109) (E5I9G) Rabbit mAb. Western blot analysis was performed using Phospho-YAP (Ser109) Antibody #46931. Mouse anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.
YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser127, Ser397) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (6-8). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (9).
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