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#53749 Phospho-YAP (Ser109) (E5I9G) Rabbit mAb

 

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2019年1月18日15時10分 現在
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YAP 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性78Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

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53749 の推奨プロトコール i

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特異性・感度
Phospho-YAP (Ser109) (E5I9G) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser109. Based on sequence similarity, the antibody may recognize TAZ protein when phosphorylated at Ser66.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser109 of human YAP protein.

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社内データ

※下記の社内データは、すべて53749 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1 cells and HeLa cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ-phosphatase (+), using Phospho-YAP (Ser109) (E5I9G) Rabbit mAb (upper), YAP (D8H1X) XP® Rabbit mAb #14074 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using using Phospho-YAP (Ser109) (E5I9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). RL-7 cells are negative for YAP expression, confirming specificity of the antibody. YAP expression is undetectable in RL-7 extracts, as predicted from published gene expression databases and as confirmed with other YAP antibodies.

IP

IP

Immunoprecipitation of Phospho-YAP (Ser109) protein from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-YAP (Ser109) (E5I9G) Rabbit mAb. Western blot analysis was performed using Phospho-YAP (Ser109) Antibody #46931. Mouse anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.


バックグラウンド

YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser127, Ser397) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (6-8). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (9).

使用例
 
関連製品
14074   YAP (D8H1X) XP® Rabbit mAb
4912   YAP Antibody
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Phospho-YAP (Ser109) (E5I9G) Rabbit mAb

Metabolic Reprogramming in Disease

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