#54837 FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 488 Conjugate)
|FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total FcγRIIB protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro284 of mouse FcγRIIB protein.|
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Flow cytometric analysis of mouse splenocytes using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 488 Conjugate) (right) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (left). Samples were co-stained with CD45R (B220)-APC to distinguish the B cell population (top) or CD3-APC to distinguish the T cell population (bottom).
FcγRIIB (CD32B) is a low affinity, IgG Fc-binding receptor expressed on B cells, monocytes, macrophages, and dendritic cells (DCs) (1-3). It is the inhibitory Fc receptor and signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) within its carboxy-terminal cytoplasmic tail (2). Binding of immune complexes to FcγRIIB results in tyrosine phosphorylation of the ITIM motif at Tyr292 and recruitment of the phosphatase SHIP, which mediates inhibitory effects on immune cell activation (2,4). In this way, FcγRIIB suppresses the effects of activating Fc-binding receptors (3). For example, mice deficient for FcγRIIB have greater T cell and DC responses following injection of immune complexes (5, 6). In addition, FcγRIIB plays a role in B cell affinity maturation (7). Signaling through FcγRIIB in the absence of signaling through the B cell receptor (BCR) is proapoptotic, while signaling through FcγRIIB and the BCR simultaneously attenuates the apoptotic signal and results in selection of B cells with higher antigen affinity (7).
- Tridandapani, S. et al. (2002) J. Biol. Chem. 277, 5082-5089.
- Tridandapani, S. et al. (1997) Mol. Cell. Biol. 17, 4305-4311.
- Guilliams, M. et al. (2014) Nat Rev Immunol 14, 94-108.
- Bruhns, P. et al. (2000) J. Biol. Chem. 275, 37357-37364.
- Kalergis, A.M. and Ravetch, J.V. (2002) J Exp Med 195, 1653-9.
- Desai, D.D. et al. (2007) J Immunol 178, 6217-26.
- Pearse, R.N. et al. (1999) Immunity 10, 753-60.
|96397 FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific)|
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