#55948 NADK Antibody
|ヒトのNADK タンパク質のN末端近傍領域 (合成ペプチド)|
ホモロジー (相同性) 検索をご希望の場合 >>>
Western blot analysis of extracts from 293 cells, transfected with non-targeted (-) or targeted (+) siRNA, using NADK Antibody (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Immunoprecipitation of NADK from COS-7 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is NADK Antibody. Western blot analysis was performed using NADK Antibody. Anti-Rabbit light chain-specific secondary antibody was used for detection.
NADK, also known as NAD kinase, is a cytoplasmic protein responsible for maintaining the pool of available NADP+ and NADPH within the cell (1). Using ATP as a phosphate donor, NADK catalyzes the phosphorylation of NAD+ to NADP+. This molecule is then reduced to NADPH and utilized in various metabolic and biosynthetic pathways (2). NADK has been suggested to play a role in glucose metabolism due to the effect NADPH production has on both the insulin secretion and survival of pancreatic β-cells (3). NADPH has a vital role in protecting cells from oxidative stress through its neutralizing effect on reactive oxygen species (ROS), which also accumulate during cell growth (2, 3, 4). Along with the p53 tumor suppression protein, NADK has been a suggested target in cancer therapy due its link to NADPH production and its resulting protective role on growing and proliferating cells (2).
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.