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#56674 YAP/TAZ Transcriptional Targets Antibody Sampler Kit

 
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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
CTGF (D8Z8U) Rabbit mAb #86641 20 µl WB, IF-IC H 35 Rabbit IgG
CYR61 (D4H5D) XP® Rabbit mAb #14479 20 µl WB, IF-IC, F H 41 Rabbit IgG
IGFBP3 (D1U9C) Rabbit mAb #25864 20 µl WB, IP H 40 Rabbit IgG
Integrin β2 (D4N5Z) Rabbit mAb #73663 20 µl WB H 95 Rabbit IgG
Survivin (71G4B7) Rabbit mAb #2808 20 µl WB, IP, IHC-P, IHC-F, IF-IC, F H, M, R 16 Rabbit IgG
Axl (C89E7) Rabbit mAb #8661 20 µl WB, IP, IHC-P, IF-IC, F H, Mk 138 Rabbit IgG
Pan-TEAD (D3F7L) Rabbit mAb #13295 20 µl WB, IP H, M, R, Mk 50, 53, 55, 60 Rabbit IgG
Lamin B2 (D8P3U) Rabbit mAb #12255 20 µl WB, IP, IF-IC H, Mk 68 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Axl (C89E7) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using TEAD1 (D9X2L) Rabbit mAb #12292 (A), TEAD2 Antibody #8870 (B), TEAD3 Antibody (C), or Pan-TEAD (D3F7L) Rabbit mAb #13295 (D).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CYR61 (D4H5D) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MCF7 cells are negative for CYR61 expression.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Integrin β2 (D4N5Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CTGF (D8Z8U) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

Western Blotting

Western Blotting

Western blot analysis of extracts from concentrated culture medium of various cell lines using IGFBP3 (D1U9C) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded breast carcinoma using Axl (C89E7) Rabbit mAb. Note staining of infiltrating cells.

IP

IP

Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using Pan-TEAD (D3F7L) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293T cells (blue) and PANC-1 cells (green) using CYR61 (D4H5D) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of PANC-1 cells (high-expressing) and MCF7 cells (low-expressing) using CTGF (D8Z8U) Rabbit mAb (green) and an Anti-Na+/K+ ATPase α Mouse mAb (blue). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.


IP

IP

Immunoprecipitation of IGFBP3 protein from HCC1937 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IGFBP3 (D1U9C) Rabbit mAb. Western blot analysis was performed using IGFBP3 (D1U9C) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded metastatic lung carcinoma using Axl (C89E7) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pan-TEAD (D3F7L) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Saos-2 cells, untreated (left) or treated with Brefeldin A #9972 (10 μg/ml, overnight; center), and untreated MCF7 cells (right), using CYR61 (D4H5D) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4804 (fluorescent DNA dye).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, NCI-H1299 (left) or Jurkat (right), using Axl (C89E7) Rabbit mAb.

IP

IP

Immunoprecipitation of TEAD proteins from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Pan-TEAD (D3F7L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Pan-TEAD (D3F7L) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and DU145 cells (green) using Axl (C89E7H4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of DU 145 (left) and HCC827 (right) cells using Axl (C89E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with DNA-binding transcription factors (e.g., Transcriptional Enhanced Activation Domain [TEAD] proteins) to regulate the expression of genes that control fundamental aspects of cell function, such as proliferation and cell survival (1). A number of genes have been experimentally confirmed as targets of transcriptional regulation by YAP and TAZ. These include the extracellular matrix proteins CTGF, CYR61, and integrin β2 (2-4), the inhibitor of apoptosis protein (IAP) survivin (5), the mechano-sensitive nuclear envelope protein Lamin B2 (6), and the oncogenic receptor tyrosine kinase Axl (7).

使用例
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関連製品
14074   YAP (D8H1X) XP® Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
70148   TAZ (D3I6D) Rabbit mAb
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

YAP/TAZ Transcriptional Targets Antibody Sampler Kit

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