#57246 PTBP1 (E4I3Q) Rabbit mAb
|内在性||57, 59||Rabbit IgG||-20℃|
|PTBP1 (E4I3Q) Rabbit mAb recognizes endogenous levels of total PTBP1 protein. This antibody does not cross-react with PTBP2 protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val10 of human PTBP protein.|
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Western blot analysis of extracts from various cell lines using PTBP1 (E4I3Q) Rabbit mAb.
Western blot analysis of extracts from 293T cells, either mock transfected (-), transfected (+) with constructs expressing Myc/DDK-tagged full-length human PTBP1 (hPTBP1-Myc/DDK) or Myc/DDK-tagged full-length human PTBP2 (hPTBP2-Myc/DDK), using PTBP1 (E4I3Q) Rabbit mAb (upper) or DYKDDDDK Tag (D6W5B) Rabbit mAb #14793 (lower).
Immunoprecipitation of PTBP1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PTBP1 (E4I3Q) Rabbit mAb. Western blot analysis was performed using PTBP1 (E4I3Q) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min) and either PTBP1 (E4I3Q) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human LENG8 5' UTR Primers #70823 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
PTBP1 and PTBP2 are highly related pre-mRNA binding proteins that silence the splicing of alternative exons (1,2 ). While PTBP1 is widely expressed, PTBP2, also known as neural PTB or nPTB, is expressed in post-mitotic neurons. In other cell types, PTBP2 transcripts are alternatively spliced by PTBP1, which leads to nonsense-mediated decay (3). PTBP1 and 2 bind to similar regions across the transcriptome, but PTBP1 has a stronger repressive property likely due to cofactors such as Raver1 and Matrin3 (4-6). Overexpression of PTBPs in various cancers has been observed, leading to alternative splicing of key proteins in oncogenic pathways (6-9).
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Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.