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#57295 PhosphoPlus® Rictor (Thr1135) Antibody Duet

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月22日15時35分 現在
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#57295S1 Kit106,000

Rictor 製品一覧

57295 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。


キット内容 容量 用途 種交差性 Mol. Wt. アイソタイプ
Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb #3806 100 µl WB H, M, Mk 200 kDa Rabbit IgG
Rictor (53A2) Rabbit mAb #2114 100 µl WB H, M, R, Mk 200 kDa Rabbit IgG

Applications Key: W=Western Blotting

Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Rictor (53A2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved HeLa cells, untreated or treated with IGF-1 (50 ng/ml, 30 minutes) and λ phosphatase, using Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb (upper) or Rictor (53A2) Rabbit mAb #2114 (lower).


Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity (6-8).

1072   mTOR Blocking Peptide
1230   Phospho-mTOR (Ser2448) Blocking Peptide
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2083   Phospho-Raptor (Ser792) Antibody
2114   Rictor (53A2) Rabbit mAb
2140   Rictor Antibody
2280   Raptor (24C12) Rabbit mAb
2971   Phospho-mTOR (Ser2448) Antibody
2972   mTOR Antibody
2974   Phospho-mTOR (Ser2481) Antibody
2976   Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
2983   mTOR (7C10) Rabbit mAb
3806   Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb
4517   mTOR (L27D4) Mouse mAb
5536   Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb
6381   SignalSilence® mTOR siRNA I
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9862   mTOR Substrates Antibody Sampler Kit
9904   Rapamycin
9964   mTOR Pathway Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

CST is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.


PhosphoPlus® Rictor (Thr1135) Antibody Duet

Immune Cell Signaling Pathways