#58121 MGMT (E6M7V) Rabbit mAb
|MGMT (E6M7V) Rabbit mAb recognizes endogenous levels of total MGMT protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly200 of human MGMT protein.|
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Western blot analysis of extracts from various cell lines using MGMT (E6M7V) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MDA-MB-231 cells do not express MGMT.
Immunoprecipitation of MGMT from VCAP cell extracts. Lane 1 is 10% input, lane is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MGMT (E6M7V) Rabbit mAb. Western blot analysis was performed using MGMT (E6M7V) Rabbit mAb.
Confocal immunofluorescent analysis of MCF7 cells (left, positive) and SW620 cells (right, negative) using MGMT (E6M7V) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O(6) position of guanine, restoring guanine to its normal form without causing DNA breaks (1). MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents (2,3). It is ubiquitously expressed in normal human tissues (4) and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy (5).
|7003 20X LumiGLO® Reagent and 20X Peroxide|
|7071 Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody|
|7074 Anti-rabbit IgG, HRP-linked Antibody|
|7727 Biotinylated Protein Ladder Detection Pack|
|9999 Nonfat Dry Milk|
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.