#5887 γ Secretase Antibody Sampler Kit
|Nicastrin (D38F9) Rabbit mAb #5665||20 µl||WB||H, M, R, Hm, Mk||110, 120||Rabbit IgG|
|PEN2 (D6G8) Rabbit mAb #8598||20 µl||WB, IP||H, M, R, Mk||13||Rabbit IgG|
|Presenilin 1 (D39D1) Rabbit mAb #5643||20 µl||WB, IP||H, M, R, Mk||22 (CTF)||Rabbit IgG|
|Presenilin 2 (D30G3) Rabbit mAb #9979||20 µl||WB, IP||H, M, R||23 (CTF)||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||WB||Goat|
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell lines using Nicastrin (D38F9) Rabbit mAb.
Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).
Western blot analysis of extracts from Presenilin 1 (-/-) and Presenilin 2 (-/-) MEF cells using Presenilin 2 (D30G3) Rabbit mAb (upper) and Presenilin 1 (D39D1) Rabbit mAb #5643 (lower). (Presenilin 1 (-/-) and Presenilin 2 (-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).
The γ secretase protease complex interacts with and cleaves intramembrane substrates as an essential function for regulation of intracellular signaling and cell-cell interactions. This multiprotein complex is comprised of four integral membrane proteins, Presenilin, Nicastrin, Aph-1, and PEN2, all of which are essential for complete proteolytic activity (1). Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin’s associated γ-secretase activity for amyloid-β protein processing (2,3). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (3,4). Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (5,6). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (7). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to γ-secretase to ensure the correct cleavage process (6,8). Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (9). PEN2 is an important part of the γ-secretase complex as its knock down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (10).
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LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
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