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#5887 γ Secretase Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#5887T1 Kit75,000
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Nicastrin (D38F9) Rabbit mAb #5665 20 µl WB H, M, R, Hm, Mk 110, 120 Rabbit IgG
PEN2 (D6G8) Rabbit mAb #8598 20 µl WB, IP H, M, R, Mk 13 Rabbit IgG
Presenilin 1 (D39D1) Rabbit mAb #5643 20 µl WB, IP H, M, R, Mk 22 (CTF) Rabbit IgG
Presenilin 2 (D30G3) Rabbit mAb #9979 20 µl WB, IP H, M, R 23 (CTF) Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Nicastrin (D38F9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).


Western Blotting

Western Blotting

Western blot analysis of extracts from Presenilin 1 (-/-) and Presenilin 2 (-/-) MEF cells using Presenilin 2 (D30G3) Rabbit mAb (upper) and Presenilin 1 (D39D1) Rabbit mAb #5643 (lower). (Presenilin 1 (-/-) and Presenilin 2 (-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PEN2 (D6G8) Rabbit mAb.

バックグラウンド

The γ secretase protease complex interacts with and cleaves intramembrane substrates as an essential function for regulation of intracellular signaling and cell-cell interactions. This multiprotein complex is comprised of four integral membrane proteins, Presenilin, Nicastrin, Aph-1, and PEN2, all of which are essential for complete proteolytic activity (1). Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin’s associated γ-secretase activity for amyloid-β protein processing (2,3). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (3,4). Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (5,6). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (7). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to γ-secretase to ensure the correct cleavage process (6,8). Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (9). PEN2 is an important part of the γ-secretase complex as its knock down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (10).

使用例
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LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

γ Secretase Antibody Sampler Kit

Immune Cell Signaling Pathways

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