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#59973 HIF-2α (D6T8V) Rabbit mAb

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2019年3月22日15時35分 現在
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#59973S100 μL56,000

HIF2A 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性120Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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59973 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)クロマチン免疫沈降 (1:100)


HIF-2α (D6T8V) Rabbit mAb recognizes endogenous levels of total HIF-2α protein. This antibody does not cross-react with HIF-1α protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly688 of human HIF-2α protein.

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※下記の社内データは、すべて59973 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 786-O and A498 cells using HIF-2α (D6T8V) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of Hep G2 cells, untreated (-) or treated with cobalt chloride (CoCl2, 100 μM, 4 hr, +) using HIF-2α (D6T8V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from Hep3B2.1-7 cells treated with cobalt chloride (100 μM) overnight and either HIF-2α (D6T8V) Rabbit mAb #59973 or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PAI-1 Promoter Primers #33070, human EPO promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits the degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
14179   HIF-1α (D2U3T) Rabbit mAb
33070   SimpleChIP® Human PAI-1 Promoter Primers
3414   HIF-1β/ARNT (C15A11) Rabbit mAb
3434   Hydroxy-HIF-1α (Pro564) (D43B5) XP® Rabbit mAb
36169   HIF-1α (D1S7W) XP® Rabbit mAb
3716   HIF-1α Antibody
4486   SimpleChIP® Human α Satellite Repeat Primers
5537   HIF-1β/ARNT (D28F3) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7096   HIF-2α (D9E3) Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
79233   HIF-1α (D5F3M) Mouse mAb
88989   SimpleChIP® Universal qPCR Master Mix
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


HIF-2α (D6T8V) Rabbit mAb

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