The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.
F(ab')2 fragments are prepared from goat antibodies that have been affinity purified and shown no reactivity against non-immunoglobulin mouse serum proteins or bovine, goat, human, rabbit or rat IgG based on immunoelectrophoresis (IEP) testing.
Flow cytometric analysis of Jurkat cells using β-Actin (8H10D10) Mouse mAb #3700 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) (solid line) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line).
This product has been optimized for use as a secondary antibody in flow cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
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