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#60968 ZBP1 Antibody

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2019年3月22日15時35分 現在
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#60968S100 μL56,000
感度分子量 (kDa)抗体の由来貯法
内在性44, 58Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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60968 の推奨プロトコール i

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ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


ZBP1 Antibody recognizes endogenous levels of total ZBP1 protein. A band of unknown identity is detected at around 19 kDa.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln372 of human ZBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

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※下記の社内データは、すべて60968 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ZBP1 (hZBP1-Myc/DDK; +), using ZBP1 Antibody (upper) or Myc Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ZBP1 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Based on RNA-seq data, ZBP1 is predicted not to be expressed in A549 cells.



Immunoprecipitation of ZBP1 from NCI-H929 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is ZBP1 Antibody. Western blot was performed using ZBP1 Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP-Conjugate) #5127 was used as a secondary antibody.


ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide binding protein that plays a role in tumorigenesis and innate immune responses to viral infection (1). It is expressed at high levels in lymphatic tissues and intestine and is induced in macrophages by interferon gamma or by LPS (2,3). It contains two amino terminal Z-alpha domains that bind to left-handed Z-DNA and Z-RNA (4,5). In addition, an adjacent domain binds right-handed B-DNA that allows for it to function as a cytosolic DNA sensor in innate immune responses, triggering activation of TBK1 and IRF-3, and subsequent production of type I interferons (6,7). Furthermore, ZBP1 can trigger the activation of NF-κB through interaction with the RIP homotypic interaction motif (RHIM) of RIPK1 and RIPK3 (8). ZBP1 binding to RIPK3 can also induce a process of programmed necrosis termed necroptosis (9). In contrast, its interaction with RIPK1 can repress necroptosis (10,11). The mRNA binding activity of ZBP1 is also thought to play a role in tumorigenesis. ZBP1 is repressed in metastatic breast cancer, which leads to dysregulation of mRNA targets involved in proliferation and metastasis (12,13).

12630   SignalFire™ Plus ECL Reagent
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6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

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ZBP1 Antibody

Metabolic Reprogramming in Disease