#60968 ZBP1 Antibody
|ZBP1 Antibody recognizes endogenous levels of total ZBP1 protein. A band of unknown identity is detected at around 19 kDa.|
|Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln372 of human ZBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.|
ホモロジー (相同性) 検索をご希望の場合 >>>
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ZBP1 (hZBP1-Myc/DDK; +), using ZBP1 Antibody (upper) or Myc Tag (71D10) Rabbit mAb #2278 (lower).
Western blot analysis of extracts from various cell lines using ZBP1 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Based on RNA-seq data, ZBP1 is predicted not to be expressed in A549 cells.
Immunoprecipitation of ZBP1 from NCI-H929 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is ZBP1 Antibody. Western blot was performed using ZBP1 Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP-Conjugate) #5127 was used as a secondary antibody.
ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide binding protein that plays a role in tumorigenesis and innate immune responses to viral infection (1). It is expressed at high levels in lymphatic tissues and intestine and is induced in macrophages by interferon gamma or by LPS (2,3). It contains two amino terminal Z-alpha domains that bind to left-handed Z-DNA and Z-RNA (4,5). In addition, an adjacent domain binds right-handed B-DNA that allows for it to function as a cytosolic DNA sensor in innate immune responses, triggering activation of TBK1 and IRF-3, and subsequent production of type I interferons (6,7). Furthermore, ZBP1 can trigger the activation of NF-κB through interaction with the RIP homotypic interaction motif (RHIM) of RIPK1 and RIPK3 (8). ZBP1 binding to RIPK3 can also induce a process of programmed necrosis termed necroptosis (9). In contrast, its interaction with RIPK1 can repress necroptosis (10,11). The mRNA binding activity of ZBP1 is also thought to play a role in tumorigenesis. ZBP1 is repressed in metastatic breast cancer, which leads to dysregulation of mRNA targets involved in proliferation and metastasis (12,13).
- Kuriakose, T. and Kanneganti, T.D. (2018) Trends Immunol 39, 123-34.
- Rothenburg, S. et al. (2002) Nucleic Acids Res 30, 993-1000.
- Fu, Y. et al. (1999) Gene 240, 157-63.
- Ross, A.F. et al. (1997) Mol Cell Biol 17, 2158-65.
- Schwartz, T. et al. (2001) Nat Struct Biol 8, 761-5.
- Takaoka, A. et al. (2007) Nature 448, 501-5.
- Wang, Z. et al. (2008) Proc Natl Acad Sci U S A 105, 5477-82.
- Rebsamen, M. et al. (2009) EMBO Rep 10, 916-22.
- Upton, J.W. et al. (2012) Cell Host Microbe 11, 290-7.
- Newton, K. et al. (2016) Nature 540, 129-33.
- Lin, J. et al. (2016) Nature 540, 124-8.
- Gu, W. et al. (2009) J Cell Sci 122, 1895-905.
- Wang, G. et al. (2016) Oncotarget 7, 15690-702.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.