#61237 PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 647 Conjugate)
|PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PD-1 protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala242 of mouse PD-1 protein.|
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Confocal immunofluorescent analysis of lymph node from a syngeneic CT26 tumor-bearing mouse. Sections were labeled with PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 647) (red) and Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882 (green) before mounting with Prolong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of EL-4 cells (left, positive) and NIH/3T3 (right, negative) using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 647 Conjugate) (red). Blue = Hoechst 33342 #4082 (fluorescent DNA dye).
The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).
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- Dong, H. et al. (2002) Nat Med 8, 793-800.
- Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.
|84651 PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific)|
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XP is a registered trademark of Cell Signaling Technology, Inc.
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