#64718 MLANA/MART-1 (E9Q4O) XP® Rabbit mAb
IHC-P: 抗体希釈液 / 抗原賦活化
|MLANA/MART-1 (E9Q4O) Rabbit mAb recognizes endogenous levels of total MLANA/MART-1 protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLANA/MART-1 protein.|
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Western blot analysis of extracts from various cell lines using MLANA/MART1 (E9Q4O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Expression in OVCAR3 is negative or low, as expected.
Immunoprecipitation of MLANA/MART1 protein from SK-MEL-28 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MLANA/MART1 (E9Q4O) XP® Rabbit mAb. Western blot analysis was performed using MLANA/MART1 (E9Q4O) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 cell pellet (left, positive) or OVCAR3 cell pellet (right, negative) using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human melanoma (left, positive) or colon carcinoma (right, negative) using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human skin using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb.
Confocal immunofluorescent analysis of SK-MEL-2 (left, positive) and OVCAR3 (right, negative) using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
MLANA, also known as MART-1, is a member of a melanocyte lineage-specific family of proteins. It is expressed in melanocytes, retinal pigment epithelium, and melanoma cells. Its function is not entirely understood, but it is believed to be involved in the stability of GPR143, as well as the stability, trafficking, and processing of PMEL; both proteins are involved in the formation of stage II melanosomes (1). In melanosomes, MLANA is specifically located in the trans-Golgi network, however conformational changes to the protein or a sub-population of the protein causes it to localize back to the ER and small endosomal vesicles (2). In the context of melanoma cells, the conformational change is thought to be caused by aberrant exposure of epitopes, which are recognized by cytolytic T-lymphocytes (3). MLANA may be useful as a marker of metastatic melanoma (4). MHC-II restricted phospho-MLANA peptides, which are recognized by CD4 cells, are being investigated as potential candidates for cancer immunotherapy (5).
DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.