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#64718 MLANA/MART-1 (E9Q4O) XP® Rabbit mAb

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CSTコード 包装
希望納入価格 (円)
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国内在庫 i
2019年3月22日15時35分 現在
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#64718S100 μL62,000
#64718T20 μL39,000

MLANA 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性19Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト -
64718 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:200)免疫蛍光細胞染色 (IF-IC) (1:800)


IHC-P: 抗体希釈液 / 抗原賦活化


MLANA/MART-1 (E9Q4O) Rabbit mAb recognizes endogenous levels of total MLANA/MART-1 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLANA/MART-1 protein.

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※下記の社内データは、すべて64718 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MLANA/MART1 (E9Q4O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Expression in OVCAR3 is negative or low, as expected.



Immunoprecipitation of MLANA/MART1 protein from SK-MEL-28 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MLANA/MART1 (E9Q4O) XP® Rabbit mAb. Western blot analysis was performed using MLANA/MART1 (E9Q4O) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-MEL-28 cell pellet (left, positive) or OVCAR3 cell pellet (right, negative) using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human melanoma (left, positive) or colon carcinoma (right, negative) using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skin using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb.



Confocal immunofluorescent analysis of SK-MEL-2 (left, positive) and OVCAR3 (right, negative) using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


MLANA, also known as MART-1, is a member of a melanocyte lineage-specific family of proteins. It is expressed in melanocytes, retinal pigment epithelium, and melanoma cells. Its function is not entirely understood, but it is believed to be involved in the stability of GPR143, as well as the stability, trafficking, and processing of PMEL; both proteins are involved in the formation of stage II melanosomes (1). In melanosomes, MLANA is specifically located in the trans-Golgi network, however conformational changes to the protein or a sub-population of the protein causes it to localize back to the ER and small endosomal vesicles (2). In the context of melanoma cells, the conformational change is thought to be caused by aberrant exposure of epitopes, which are recognized by cytolytic T-lymphocytes (3). MLANA may be useful as a marker of metastatic melanoma (4). MHC-II restricted phospho-MLANA peptides, which are recognized by CD4 cells, are being investigated as potential candidates for cancer immunotherapy (5).

13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.


MLANA/MART-1 (E9Q4O) XP® Rabbit mAb

Metabolic Reprogramming in Disease