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#64815 PhosphoPlus® PRAS40 (Thr246) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
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2019年3月22日15時35分 現在
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PRAS40 製品一覧

64815 の推奨プロトコール i

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キット内容 容量 用途 種交差性 Mol. Wt. アイソタイプ
Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997 100 µl WB, IP, IHC-P H, M, R, Mk 40 kDa Rabbit IgG
PRAS40 (D23C7) XP® Rabbit mAb #2691 100 µl WB, IP, IHC-P H, M, R, Mk 40 kDa Rabbit IgG

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)

Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PRAS40 (D23C7) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved H3255, Mkn45 and NIH/3T3 cells, untreated or treated with either Gefitinib (1 μM, 3 hours), Su11274 (1 μM, 3 hours) or insulin (150 nM, 15 minutes), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 (D23C7) Rabbit mAb #2691 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PRAS40 (D23C7) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with insulin (100 nM, 5 minutes) or with insulin and λ phosphatase, using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded metastatic SKOV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

バックグラウンド

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12860   Sin1 (D7G1A) Rabbit mAb
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14716   Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
2083   Phospho-Raptor (Ser792) Antibody
2114   Rictor (53A2) Rabbit mAb
2140   Rictor Antibody
2280   Raptor (24C12) Rabbit mAb
2610   PRAS40 Antibody
2640   Phospho-PRAS40 (Thr246) Antibody
2691   PRAS40 (D23C7) XP® Rabbit mAb
2971   Phospho-mTOR (Ser2448) Antibody
2972   mTOR Antibody
2974   Phospho-mTOR (Ser2481) Antibody
2983   mTOR (7C10) Rabbit mAb
2997   Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb
3227   GβL Antibody
3274   GβL (86B8) Rabbit mAb
5536   Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8101   SignalSlide® Phospho-Akt (Ser473) IHC Controls
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9864   mTOR Regulation Antibody Sampler Kit
9964   mTOR Pathway Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

CST is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

PhosphoPlus® PRAS40 (Thr246) Antibody Duet

Immune Cell Signaling Pathways

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