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#68014 GITR (D9I9D) Rabbit mAb (IHC Preferred)


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2019年3月26日15時45分 現在
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#68014S100 μL58,000
#68014T20 μL   for Custom Sampler Kit
Custom Antibody Sampler Kitの構成品を選択できます。

TNFRSF18 製品一覧 | #68014 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性25Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト サル
68014 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫組織染色 (パラフィン) (1:400)Immunohistochemistry (Leica® Bond™) (1:400)


IHC-P: 抗体希釈液 / 抗原賦活化


内在性レベルのGITR タンパク質を検出します。
ヒトのGITR タンパク質のVal142 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて68014 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from human CD8+ T cells, HuT 102, and Jurkat cells using GITR (D9I9D) Rabbit mAb (IHC Preferred) (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD8+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using GITR (D9I9D) Rabbit mAb (IHC Preferred) performed on the Leica® BOND™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using GITR (D9I9D) Rabbit mAb (IHC Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human HuT 102 (left) and Jurkat (right) cell pellets using GITR (D9I9D) Rabbit mAb (IHC Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using GITR (D9I9D) Rabbit mAb (IHC Preferred).


TNFRSF18, also known as glucocorticoid-induced tumor necrosis factor-receptor (TNFR)-related protein (GITR) and activation-inducible TNFR family receptor, encodes a type 1 membrane protein of the TNF-receptor superfamily (1). Three alternatively spliced transcript variants encoding distinct isoforms have been reported (2). GITR is an immune cell co-stimulatory receptor expressed constitutively at high levels on CD4+CD25+ T regulatory cells (Tregs), at low levels on naive and memory T cells, and is induced upon T cell activation (3-5). Studies show GITR can also be induced on NK cells, macrophages, and DCs (3, 4, 6). Although GITR does not have intrinsic enzymatic activity, TNFSF18 (also known as GITRL) expressed on antigen presenting cells binds to GITR resulting in recruitment of TNFR-associated factor family members and activation of the NF-kappa-B pathway in T cells (7). GITR ligation has been shown to play a role in CD8+ T cell activation, cytoxicity, and memory T cell survival (8-10). In the thymus, GITR is thought to play a key role in dominant immunological self-tolerance through thymic Treg differentiation and expansion (11). Of note, GITR ligation inhibits Treg suppressive function (12-13) and promotes effector T cell resistance to Treg suppression (14-15). Due to the combined effects on both Treg suppression and effector cell activation, GITR represents a unique opportunity for immunotherapeutic intervention in cancer (16).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
6883   SignalFire™ ECL Reagent
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


GITR (D9I9D) Rabbit mAb (IHC Preferred)

Metabolic Reprogramming in Disease