#68978 MAGE-A10 Antibody
|内在性レベルのMAGE-A10 タンパク質を検出します。他のMAGE-A ファミリータンパク質とは交差しません。|
|ヒトのMAGE-A10 タンパク質のPro323 周辺領域 (合成ペプチド)|
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Western blot anlysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human MAGE-A2 (hMAGE-A2-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A3 (hMAGE-A3-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A4 (hMAGE-A4-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A6 (hMAGE-A6-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A10 (hMAGE-A10-Myc/DDK), and Myc/DDK-tagged full-length human MAGE-A12 (hMAGE-A12-Myc/DDK), using MAGE-A10 Antibody (upper), DYKDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using MAGE-A10 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MAGE-A10 protein is not detected in MCF7 cells.
Immunoprecipitation of MAGE-A10 from SK-MEL-2 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MAGE-A10 Antibody. Western blot analysis was performed using MAGE-A10 Antibody. A HRP-conjugated anti-rabbit light chain specific secondary antibody was used for detection.
Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3).
Melanoma-associated antigen-A10 (MAGE-A10) is a cancer testis antigen and belongs to the type I MAGE family of proteins. Unlike other members of the MAGE-A subfamily, MAGE-A10 is a nuclear protein (4). Research studies have shown that MAGE-A10 expression is normally restricted to the human testis and placenta but is aberrantly upregulated in a number of human solid tumors, such as lung cancer, melanoma, bladder cancer, and esophageal carcinoma (4-6). Due to its upregulated expression in human tumors and high degree of immunogenicity, MAGE-A10 has received significant attention as a novel immunotherapy target through the use of vaccines and adoptive cell therapy (7-9).
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