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#69159 N6-mA Methyltransferase Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#69159T1 Kit75,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
METTL3 (D2I6O) Rabbit mAb #96391 20 µl WB, IP H, M, R, Mk Hm, Pg, Hr 70 Rabbit IgG
METTL14 (D8K8W) Rabbit mAb #51104 20 µl WB H, M, R, Mk C, X 65 Rabbit IgG
Virilizer (D4N8B) Rabbit mAb #88358 20 µl WB, IP, ChIP H, M, R, Mk 210 Rabbit IgG
WTAP Antibody #56501 20 µl WB, IP H, M, R, Mk 55 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using METTL3 (D2I6O) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using METTL14 (D8K8W) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Virilizer (D4N8B) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WTAP Antibody.

IP

IP

Immunoprecipitation of METTL3 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is METTL3 (D2I6O) Rabbit mAb. Western blot analysis was performed using METTL3 (D2I6O) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 secondary antibody.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from mES cells and either Virilizer (D4N8B) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Sox2 Exon 1 Primers #30180, mouse Klf2 intron 2 primers, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

Methyltransferase-like protein 3 (METTL3) and methytransferase-like protein 14 (METTL14) are the two catalytic subunits of an N6-methyltransferase complex that methylates adenosine residues in RNA (1). Methylation of adenosine residues regulates mRNA splicing, processing, translation efficiency, editing and stability, in addition to regulating primary miRNA processing, and is critical for proper regulation of the circadian clock, embryonic stem cell self-renewal, immune tolerance, response to various stimuli, meiosis and mouse fertility (2,3). In this complex, METTL3 functions as the catalytic methyltransferase subunit and METTL14 functions as the target recognition subunit by binding to RNA (4). In addition, the Wilms tumor 1-associated protein (WTAP) functions as a regulatory subunit and is required for accumulation of the complex to nuclear speckles, which are sites of RNA processing (5). Several studies suggest a role for this complex in cancer. METTL3 expression is elevated in lung adenocarcinoma where it promotes growth, survival and invasion of human lung cancer cells (6). In addition, WTAP is over-expressed in a number of different cancers and positively regulates cell migration and invasion in glioblastoma and cholangiocarcinoma (7,8).

Virilizer is an additional protein subunit of the METTL3/METTL14 N6-methyltransferase complex and is required for methyltransferase activity (9). As part of the WTAP complex, Virilizer functions to regulate sex determination in Drosophila melanogaster through splicing of Sex-lethal (Sxl) mRNA transcripts (10,11).

使用例
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関連製品
4499   Histone H3 (D1H2) XP® Rabbit mAb
51104   METTL14 (D8K8W) Rabbit mAb
56501   WTAP Antibody
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
88358   Virilizer (D4N8B) Rabbit mAb
96391   METTL3 (D2I6O) Rabbit mAb
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

N6-mA Methyltransferase Antibody Sampler Kit

Immune Cell Signaling Pathways

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