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#72335 DNMT3B (E2Q3Z) Rabbit mAb

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2019年3月22日15時35分 現在
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#72335S100 μL56,000

DNMT3B 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性96Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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72335 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



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DNMT3B (E2Q3Z) Rabbit mAb recognizes endogenous levels of total DNMT3B protein. This antibody does not cross react with DNMT3A or DNMT1.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu563 of human DNMT3B protein.

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※下記の社内データは、すべて72335 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, Huh7, and COS-7 cell lines using DNMT3B (E2Q3Z) Rabbit mAb.



Immunoprecipitation of DNMT3B from NCCIT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DNMT3B (E2Q3Z) Rabbit mAb. Western blot analysis was performed using DNMT3B (E2Q3Z) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NCCIT cells (green), HCT116 WT cells (red), or HCT116 DNMT3B KO cells (blue), using DNMT3A (E2Q3Z) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines).

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either DNMT3B (E2Q3Z) Rabbit mAb #72335 or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human EIF4G1 Intron 8 Primers #29118, human CROCC intron 1 primers, and SimpleChIP® Human GAPDH Promoter Primers #4471. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).

13326   DMAP1 Antibody
13451   DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific)
19115   DMAP1 (D4O2G) Rabbit mAb
29118   SimpleChIP® Human EIF4G1 Intron 8 Primers
32578   DNMT3A (D2H4B) Rabbit mAb
3598   DNMT3A (D23G1) Rabbit mAb
4471   SimpleChIP® Human GAPDH Promoter Primers
48488   DNMT3B (E4I4O) Rabbit mAb (Mouse Specific)
49768   DNMT3A (E9P2F) Rabbit mAb
5032   DNMT1 (D63A6) XP® Rabbit mAb
57868   DNMT3B (E8A8A) XP® Rabbit mAb
67259   DNMT3B (D7O7O) Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
88989   SimpleChIP® Universal qPCR Master Mix
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


DNMT3B (E2Q3Z) Rabbit mAb

Immune Cell Signaling Pathways