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#73959 Matrix Remodeling Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#73959T1 Kit107,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
MMP-9 (D6O3H) XP® Rabbit mAb #13667 20 µl WB, IHC-P, F H 84, 92 Rabbit IgG
MMP-2 (D2O4T) Rabbit mAb #87809 20 µl WB, IP H, M R 64,72 Rabbit IgG
MT1-MMP (D1E4) Rabbit mAb #13130 20 µl WB H, M 50, 62 Rabbit IgG
MMP3 (D7F5B) Rabbit mAb #14351 20 µl WB H, R 60 Rabbit IgG
TIMP1 (D10E6) Rabbit mAb #8946 20 µl WB H, Mk 26 Rabbit IgG
TIMP2 (D18B7) Rabbit mAb #5738 20 µl WB H, M, Mk 22 Rabbit IgG
TIMP3 (D74B10) Rabbit mAb #5673 20 µl WB H, M, R 20, 25 Rabbit IgG
MMP-7 Antibody #71031 20 µl WB H 28 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080, A172, and COS-7 cells using TIMP2 (D18B7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431, and NIH/3T3 cells, using TIMP3 (D74B10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TIMP1 (D10E6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HT-1080, and NIH/3T3 cells using MT1-MMP (D1E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MMP3 (D7F5B) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from U87, 3T3-L1 and C2C12 cells using MMP-2 (D4O4T) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from concentrated culture medium of IGROV-1 and HT-29 cell lines using MMP-7 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of MMP-2 protein from U87 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D2O4T) Rabbit mAb. Western blot analysis was performed using MMP-2 (D2O4T) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded U-2 OS cell pellets, untreated (left) or treated with TPA #4174 (right), using MMP-9 (D6O3H) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of serum starved U-2 OS cells, untreated (blue) or treated with TPA (200nM, 24 hours) using MMP-9 (D6O3H) XP Rabbit mAb (green). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) was used as a secondary antibody.


バックグラウンド

Matrix remodeling is mainly controlled by MMPs and TIMPs. The matrix metalloproteinase (MMP) family of proteases are a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, matrix structural proteins, and other proteases (1, 2). Among the family members, MMP-2, MMP-3, MMP-7, MMP-9, and MMP14 (MT1-MMP) have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (3). MMP activity is regulated by mechanisms of both transcriptional control and post translational protein processing. Once synthesized, MMPs exist as latent proenzymes. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (4). MMP activity can be inhibited through its binding to endogenously expressed TIMPs. TIMPs are members of the family of tissue inhibitors of matrix metalloproteinases that include TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by direct binding MMPs and chelating their zinc cofactor at the catalytic site to inhibit the proteinase function (5,6).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13130   MT1-MMP (D1E4) Rabbit mAb
13667   MMP-9 (D6O3H) XP® Rabbit mAb
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14351   MMP3 (D7F5B) Rabbit mAb
5673   TIMP3 (D74B10) Rabbit mAb
5738   TIMP2 (D18B7) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
71031   MMP-7 Antibody
7727   Biotinylated Protein Ladder Detection Pack
87809   MMP-2 (D2O4T) Rabbit mAb
8946   TIMP1 (D10E6) Rabbit mAb

DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Matrix Remodeling Antibody Sampler Kit

Metabolic Reprogramming in Disease

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