CST logo

#74073 Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb

 

Shopping Days対象製品 対象製品の中から、まとめて購入で一部製品が無料に!
*ウェブで専用注文書を作成し、代理店へご注文ください。

をクリックすると選べます。

CSTコード 包装
希望納入価格 (円)
Shopping Daysで購入
国内在庫 i
2019年3月25日15時35分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#74073S100 μL66,000
ログインすると国内在庫状況がご確認いただけます。

Histone H3 製品一覧 | #74073 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル -
74073 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)クロマチン免疫沈降 (1:50)クロマチン免疫沈降シークエンス (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb recognizes endogenous levels of histone H3 protein when tri-methylated at Lys79. This antibody shows some cross-reactivity to histone H3 that is di-methylated on Lys79, but does not cross-react with non-methylated or mono-methylated histone H3 Lys79. The antibody does not cross-react with histone H3 methylated at Lys4, Lys9, Lys27, or Lys36, and does not cross-react with any other methylated histone proteins.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing histone H3 tri-methyl lysine 79.

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて74073 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across GAPDH, a known target gene of H3K79me3 (see additional figures containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human GAPDH Promoter Primers #4471, and SimpleChIP® Human MyoD1 Exon 1 Primers #4490. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


バックグラウンド

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

使用例
 
関連製品
12522   Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb
14654   12-Tube Magnetic Separation Rack
2650   Histone H3 Antibody (ChIP Formulated)
4471   SimpleChIP® Human GAPDH Promoter Primers
4490   SimpleChIP® Human MyoD1 Exon 1 Primers
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
5427   Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb
56383   SimpleChIP® Plus Sonication Chromatin IP Kit
7003   20X LumiGLO® Reagent and 20X Peroxide
7014   SimpleChIP® Human RPL30 Exon 3 Primers
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
77087   DOT1L (D1W4Z) Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
90878   DOT1L (D4O2T) Rabbit mAb
9715   Histone H3 Antibody
9717   Histone H3 (3H1) Rabbit mAb
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
CST is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications