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#74437 Fanconi Anemia Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
FANCA (D1L2Z) Rabbit mAb #14657 20 µl WB, IP H 160 Rabbit IgG
FANCB (D9W6S) Rabbit mAb #14243 20 µl WB H, M, R 90 Rabbit IgG
FANCD2 (D5L5X) Rabbit mAb #16323 20 µl WB, IP H 155, 162 Rabbit IgG
BRCA2 (D9S6V) Rabbit mAb #10741 20 µl WB H 380 Rabbit IgG
BACH1/BRIP1 Antibody #4578 20 µl WB H 145 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y, Saos-2 and Jurkat cells using BACH1/BRIP1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using FANCB (D9W6S) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FANCA (D1L2Z) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, GM06914 cells are negative for FANCA.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts fro HeLa, U-2 OS, and GM16633 (PD20, FANCD2-negative) cells using FANCD2 (D5L5X) Rabbit mAb (upper) or α-Actinin (D6S6) Rabbit mAb #6487 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human FANCB (FANCB-Myc/DDK; +) protein, using FANCB (D9W6S) Rabbit mAb.

IP

IP

Immunoprecipitation of FANCA from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FANCA (D1L2Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using FANCA (D1L2Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with mitomycin C (2 μg/mL, 24 hr), using FANCD2 (D5L5X) Rabbit mAb. DNA damage caused by mitomycin C induces monoubiquitination of FANCD2, altering its electrophoretic mobility and increasing its apparent molecular weight (4).


IP

IP

Immunoprecipitation of FANCD2 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is FANCD2 (D5L5X) Rabbit mAb. Western blot analysis was performed using FANCD2 (D5L5X) Rabbit mAb.

バックグラウンド

Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). In response to DNA damage, the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM) induces mono-ubiquitination of FANCD2 and FANCI (2).

Monoubiquitination of FANCD2 induces localization of FANCD2 to sites of DNA damage, where it interacts with BRCA1 (4). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are also recruited to sites of DNA damage. FA signaling is important in maintenance of chromosome stability and control of mitosis (3).

使用例
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関連製品
10741   BRCA2 (D9S6V) Rabbit mAb
14243   FANCB (D9W6S) Rabbit mAb
14657   FANCA (D1L2Z) Rabbit mAb
16323   FANCD2 (D5L5X) Rabbit mAb
4578   BACH1/BRIP1 Antibody
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Fanconi Anemia Antibody Sampler Kit

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