#76682 ACTL6A/BAF53A Antibody
|ACTL6A/BAF53A Antibody recognizes endogenous levels of total ACTL6A/BAF53A protein. This antibody does not cross-react with ACTL6B/BAF53B protein. This antibody does cross-react with an unidentified protein of 95kDa.|
|Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val44 of human ACTL6A/BAF53A protein. Antibodies are purified by protein A and peptide affinity chromatography.|
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Western blot analysis of extracts from various cell lines using ACTL6A/BAF53A Antibody.
Immunoprecipitation of ACTL6A/BAF53A from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is ACTL6A/BAF53A Antibody. Western blot analysis was performed using ACTL6A/BAF53A Antibody and Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 as the secondary antibody.
The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also play a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines (6-8).
ACTL6/BAF53 proteins are highly homologous, actin-related proteins found in the SWI/SNF complex (9). In addition to the canonical SWI/SNF complex, ACT6LA/BAF53a is also member of the embryonic SWI/SNF complex, known as esBAF, which plays a role in pluripotency and development (10-12). ACTL6B/BAF53b is a member of the neural specific SWI/SNF complex and facilitates binding to target genes and is involved in memory and synaptic plasticity (13-15). ACTL6/BAF53 has been shown to interact with c-Myc, where it functions as a cofactor and is important in the transformation process (16). Further studies have shown ACTL6/BAF53 is associated with EMT and transformation in various cancers (17,18).
- Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004.
- Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7.
- Sif, S. et al. (2001) Genes Dev 15, 603-18.
- Zhang, H.S. et al. (2000) Cell 101, 79-89.
- Underhill, C. et al. (2000) J Biol Chem 275, 40463-70.
- Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33.
- Medina, P.P. et al. Epigenetics 3, 64-8.
- Medina, P.P. et al. (2008) Hum Mutat 29, 617-22.
- Zhao, K. et al. (1998) Cell 95, 625-36.
- Ho, L. et al. (2009) Proc Natl Acad Sci U S A 106, 5181-6.
- Krasteva, V. et al. (2012) Blood 120, 4720-32.
- Bao, X. et al. (2013) Cell Stem Cell 12, 193-203.
- Olave, I. et al. (2002) Genes Dev 16, 2509-17.
- Vogel-Ciernia, A. et al. (2013) Nat Neurosci 16, 552-61.
- Yoo, M. et al. (2017) J Neurosci 37, 3686-3697.
- Park, J. et al. (2002) Mol Cell Biol 22, 1307-16.
- Saladi, S.V. et al. (2017) Cancer Cell 31, 35-49.
- Sun, W. et al. (2017) Oncol Rep 37, 2405-2417.
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|9999 Nonfat Dry Milk|
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