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#76733 PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 555 Conjugate)

 

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2019年2月22日15時50分 現在
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PDCD1 製品一覧

感度抗体の由来貯法
内在性Rabbit IgG4℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

マウス ラット、ハムスター
76733 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

免疫蛍光細胞染色 (IF-IC) (1:200)免疫蛍光染色 (IF-F) (1:100)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total PD-1 protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala242 of mouse PD-1 protein.

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社内データ

※下記の社内データは、すべて76733 の推奨プロトコールで実験した結果です。

IF-F

IF-F

Confocal immunofluorescent analysis of lymph node from a syngeneic CT26 tumor-bearing mouse using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 555) (red) and Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882 (green). Samples were mounted in Prolong® Gold Antifade Reagent with DAPI #8961 (blue).

IF-IC

IF-IC

Confocal immunofluorescent analysis of EL4 cells (left, positive) and NIH/3T3 cells (right, negative) using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 555 Conjugate) (green). Blue = Hoechst 33342 #4082 (fluorescent DNA dye).

バックグラウンド

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).

使用例
 
関連製品
84651   PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific)

XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

本製品は試験研究用です。

PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 555 Conjugate)

Metabolic Reprogramming in Disease

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