The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.
F(ab')2 fragments are prepared from goat antibodies that have been affinity purified and shown no reactivity against non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse and IgG from human or mouse based on immunoelectrophoresis (IEP) testing.
Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb #4691 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) (solid line) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control
This product has been optimized for use as a secondary antibody in flow cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.