CST logo

#79891 Class II HDAC Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月20日11時40分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#79891T1 Kit75,000
ログインすると国内在庫状況がご確認いただけます。

HDAC 製品一覧

79891 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
HDAC4 (D8T3Q) Rabbit mAb #15164 20 µl WB, IP H, M, R, Mk 140 Rabbit IgG
HDAC5 (D1J7V) Rabbit mAb #20458 20 µl WB, IP H, M, R, Mk 140 Rabbit IgG
HDAC6 (D21B10) Rabbit mAb #7612 20 µl WB, IP H, M, R 160 Rabbit IgG
HDAC7 (D4E1L) Rabbit mAb #33418 20 µl WB, IHC-P H, M, Mk 124 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and Jurkat cells using HDAC6 (D21B10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC4 (D8T3Q) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC5 (D1J7V) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC7 (D4E1L) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, wild-type mouse embryonic fibroblasts (MEFs), and HDAC6 (-/-) MEFs using HDAC6 (D21B10) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).


IP

IP

Immunoprecipitation of HDAC5 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HDAC5 (D1J7V) Rabbit mAb. Western blot analysis was performed using HDAC5 (D1J7V) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC7 (D4E1L) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded K-562 (left) and LNCaP (right) cell pellets using HDAC7 (D4E1L) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HDAC7 (D4E1L) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 (D4E1L) Rabbit mAb.

バックグラウンド

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
15164   HDAC4 (D8T3Q) Rabbit mAb
20458   HDAC5 (D1J7V) Rabbit mAb
33418   HDAC7 (D4E1L) Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7612   HDAC6 (D21B10) Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Class II HDAC Antibody Sampler Kit

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications