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#8107 SignalStain® Akt Pathway IHC Sampler Kit

 
  • SignalStain® Akt Pathway IHC Sampler Kit Data Sheet PDF
CSTコード 包装
希望納入価格 (円)
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2019年1月17日15時10分 現在
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#8107S1 Kit104,000
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*  受注生産につきお届けは受注後2-3週間かかります。キットに含まれるコントロールスライドの有効期限は製造後2ヶ月です。

Akt 製品一覧

キット内容 容量 Antigen Retrieval / Diluent アイソタイプ
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 40 µl Citrate / SignalStain® Antibody Diluent #8112 Rabbit IgG
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 40 µl Citrate / SignalStain® Antibody Diluent #8112 Rabbit IgG
Akt (pan) (C67E7) Rabbit mAb #4691 40 µl Citrate / SignalStain® Antibody Diluent #8112 Rabbit IgG
PTEN (D4.3) XP® Rabbit mAb #9188 40 µl Citrate / SignalStain® Antibody Diluent #8112 Rabbit IgG
*SignalStain® Antibody Diluent #8112 25 ml
SignalSlide® Akt Family IHC Controls #8115 1 Pack

*SignalStain® Antibody Diluent is supplied as a working solution and should be stored at 4ºC (packaged separately).
†Control slides should be stored at 4ºC (packaged separately).

Custom Ordering Details

The control slides that accompany this kit are cut freshly upon ordering. Please allow up to three business days for your product to be processed.

貯法
-20℃ (SignalStain® Antibody Diluent #8112 とSignalSlide® Akt Family IHC Controls #8115 は4℃)
社内データ

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cell pellets, either untreated (left) or LY294002-treated (middle) or NIH/3T3 cell pellets (right), using Phospho-Akt (Ser473) (D9E) Rabbit mAb, Akt (pan) (C67E7) Rabbit mAb, PTEN (D4.3) Rabbit mAb and Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb. Cell pellets are provided in the SignalSlide® Akt Family IHC Controls.

Schematic

Schematic

Schematic of placement of cell pellets on SignalSlide® Akt Family IHC Controls #8115.

バックグラウンド

The protein kinase Akt (also called PKB or Rac) is activated by insulin and growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (1,2). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (3) and by carboxy terminal phosphorylation at Ser473 by the mTORC2 complex composed of mammalian target of rapamycin (mTOR) in a complex with rictor and Sin1 (4,5). Akt signaling is negatively regulated via the PTEN phosphatase (6).

Akt’s several functions include inhibition of apoptosis (7-9), regulation of glycogen synthesis via GSK-3 (10), and promotion of the cell cycle (11). Akt also regulates protein synthesis by phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (mTORC1) (12). Akt also effects mTOR activity via phosphorylation and inhibition of PRAS40 (40 kDa, proline-rich protein), which binds to raptor in the mTORC1 complex and inhibits mTOR activity (13). Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (14), allowing protein synthesis to occur. Active of mTORC1 signals to p70 S6 kinase, which in turn phosphorylates S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of a subset of mRNA transcripts that encode ribosomal proteins, translation elongation factors as well as regulators of cell cycle progression (15). Important S6 ribosomal protein phosphorylation sites include Ser235, Ser236, Ser240 and Ser244 within a small carboxy-terminal region (16).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
1085   Akt (pan) Blocking Peptide
1140   Phospho-Akt (Ser473) Blocking Peptide
1220   Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide
1250   PTEN Blocking Peptide
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
4060   Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
4691   Akt (pan) (C67E7) Rabbit mAb
4858   Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
8112   SignalStain® Antibody Diluent
9188   PTEN (D4.3) XP® Rabbit mAb

DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SignalSlide is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

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