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#8109 SignalStain® Proliferation/Apoptosis IHC Sampler Kit

 
  • SignalStain® Proliferation/Apoptosis IHC Sampler Kit Data Sheet PDF
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#8109S1 Kit104,000
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*  受注生産につきお届けは受注後2-3週間かかります。キットに含まれるコントロールスライドの有効期限は製造後2ヶ月です。

キット内容 容量 Antigen Retrieval / Diluent アイソタイプ
Cleaved Caspase-3 (Asp175) Antibody #9661 40 µl Citrate / SignalStain® Antibody Diluent #8112
Phospho-Histone H3 (Ser10) Antibody #9701 40 µl Citrate / SignalStain® Antibody Diluent #8112
PCNA (PC10) Mouse mAb #2586 40 µl Citrate / SignalStain® Antibody Diluent #8112 Mouse IgG2a
Survivin (71G4B7) Rabbit mAb #2808 40 µl Citrate / SignalStain® Antibody Diluent #8112 Rabbit IgG
*SignalStain® Antibody Diluent #8112 25 ml
SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104 1 Pack

*SignalStain® Antibody Diluent is supplied as a working solution and should be stored at 4ºC (packaged separately).
†Control slides should be stored at 4ºC (packaged separately).

Custom Ordering Details

The control slides that accompany this kit are cut freshly upon ordering. Please allow up to three business days for your product to be processed.

貯法
-20℃ (SignalStain® Antibody Diluent #8112 とSignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104 は4℃)
社内データ

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. Cell pellets are provided in the SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls.

Schematic

Schematic
バックグラウンド

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments (2). Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). PCNA protein expression is a well-accepted marker of proliferation, and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies. Histone H3, H2A, H2B, and H4 make up the core histones of the nucleosome. In response to various stimuli, the amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination, which affect the accessibility of chromatin to transcription factors (8). Phosphorylation of Histone H3 at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (9-11).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
1000   Phospho-Histone H3 (Ser10) Blocking Peptide
1037   Survivin Blocking Peptide
1050   Cleaved Caspase-3 (Asp175) Blocking Peptide
2586   PCNA (PC10) Mouse mAb
2808   Survivin (71G4B7) Rabbit mAb
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
8104   SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls
8112   SignalStain® Antibody Diluent
9661   Cleaved Caspase-3 (Asp175) Antibody
9701   Phospho-Histone H3 (Ser10) Antibody

DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SignalSlide is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

SignalStain® Proliferation/Apoptosis IHC Sampler Kit

Immune Cell Signaling Pathways

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