#8109 SignalStain® Proliferation/Apoptosis IHC Sampler Kit
|キット内容||容量||Antigen Retrieval / Diluent||アイソタイプ|
|Cleaved Caspase-3 (Asp175) Antibody #9661||40 µl||Citrate / SignalStain® Antibody Diluent #8112|
|Phospho-Histone H3 (Ser10) Antibody #9701||40 µl||Citrate / SignalStain® Antibody Diluent #8112|
|PCNA (PC10) Mouse mAb #2586||40 µl||Citrate / SignalStain® Antibody Diluent #8112||Mouse IgG2a|
|Survivin (71G4B7) Rabbit mAb #2808||40 µl||Citrate / SignalStain® Antibody Diluent #8112||Rabbit IgG|
|*SignalStain® Antibody Diluent #8112||25 ml|
|†SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104||1 Pack|
*SignalStain® Antibody Diluent is supplied as a working solution and should be stored at 4ºC (packaged separately).
†Control slides should be stored at 4ºC (packaged separately).
Custom Ordering Details
The control slides that accompany this kit are cut freshly upon ordering. Please allow up to three business days for your product to be processed.
|-20℃ (SignalStain® Antibody Diluent #8112 とSignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104 は4℃)|
Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. Cell pellets are provided in the SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments (2). Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). PCNA protein expression is a well-accepted marker of proliferation, and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies. Histone H3, H2A, H2B, and H4 make up the core histones of the nucleosome. In response to various stimuli, the amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination, which affect the accessibility of chromatin to transcription factors (8). Phosphorylation of Histone H3 at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (9-11).
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DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SignalSlide is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.