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#82577 PhosphoPlus® Tau (Ser404) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
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2019年1月15日15時15分 現在
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82577 の推奨プロトコール i

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注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 Mol. Wt. アイソタイプ
Tau (D1M9X) XP® Rabbit mAb #46687 100 µl WB, IHC-P, IF-F, IF-IC H, M, R 50 to 80 kDa Rabbit IgG
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) #20194 100 µl WB, IP, IF-F, IF-IC H, M, R 50-80 kDa Rabbit IgG

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IP=Immunoprecipitation

Reactivity Key: H=Human, M=Mouse, R=Rat

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from human cortex (lane 1), neonatal mouse brain, untreated (lane 2) or phosphatase-treated (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from MEF cells (lane 1) and mouse brain (lane 2) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Tau (D1M9X) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse primary neurons using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (green). Blue pseudocolor = Hoescht 33342 #4082 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human normal appendix using Tau (D1M9X) XP® Rabbit mAb.


IF-F

IF-F

Confocal immunofluorescent analysis of Tg2576 mouse brain, untreated (left) or Lambda Protein Phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (red) and GFAP (GA5) Mouse mAb #3670 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded T-47D cell pellet (left, positive) or MDA-MB-231 cell pellet (right, negative) using Tau (D1M9X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using Tau (D1M9X) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of T-47D (positive, left) or MDA-MB-231 (negative, right) cells using Tau (D1M9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse striatum (left), hippocampus (middle), or midbrain (right) using Tau (D1M9X) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Investigators have shown that tau phosphorylation at Ser404 destabilizes microtubules and that tau is hyperphosphorylated at Ser404 in Alzheimer's disease (4-7).

使用例
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関連製品
12456   GSK-3β (D5C5Z) XP® Rabbit mAb
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12885   Phospho-Tau (Thr181) (D9F4G) Rabbit mAb
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14145   CDK5 (D1F7M) Rabbit mAb
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
20194   Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred)
2680   p35/25 (C64B10) Rabbit mAb
35834   Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC preferred)
39357   Phospho-Tau (Ser202) (D4H7E) Rabbit mAb
4019   Tau (Tau46) Mouse mAb
46687   Tau (D1M9X) XP® Rabbit mAb
4818   GSK-3α (D80D1) XP® Rabbit mAb
5558   Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
77348   Phospho-Tau (Ser214) (D1Q2X) Rabbit mAb
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8707   MAP2 (D5G1) XP® Rabbit mAb
9632   Phospho-Tau (Ser396) (PHF13) Mouse mAb
9784   Alzheimer's Disease Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

PhosphoPlus® Tau (Ser404) Antibody Duet

Metabolic Reprogramming in Disease

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