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#82951 Semaphorin-4D/CD100 (E8S8A) Rabbit mAb

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2019年3月20日11時40分 現在
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#82951S100 μL56,000

Semaphorin 4D 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性140Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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82951 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)


Semaphorin-4D/CD100 (E8S8A) Rabbit mAb recognizes endogenous levels of total Semaphorin-4D/CD100 protein. This antibody also recognizes 25 kDa and 28 kDa Semaphorin-4D/CD100 cleavage fragments containing the carboxy terminus. This antibody cross-reacts with an unidentified protein of 50 kDa in some cell extracts.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Semaphorin-4D/CD100 protein.

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※下記の社内データは、すべて82951 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Semaphorin-4D/CD100 protein (hSema4D-Myc/DDK; +), using Semaphorin-4D/CD100 (E8S8A) Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of exctracts from HDLM-2, EL4, and P815 cells, using Semaphorin-4D/CD100 (E8S8A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Semaphorin-4D/CD100 (E8S8A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, Semaphorin-4D/CD100 protein is not expressed in PC-3 cells .



Immunoprecipitation of Semaphorin-4D/CD100 from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Semaphorin-4D/CD100 (E8S8A) Rabbit mAb. Western blot analysis was performed using Semaphorin-4D/CD100 (E8S8A) Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.


Semaphorin-4D/CD100 (Sema4D) is a disulfide-linked homodimeric type 1 transmembrane glycoprotein belonging to the class IV family of membrane bound semaphorins. The extracellular domain of Sema4D contains a cysteine-rich semaphorin-like domain, an Ig-like domain, and a PSI domain (1). Research studies have suggested that the cytoplasmic domain has a signaling function as it is phosphorylated on serine residues (2). Initial studies involving Sema4D revealed that it was implicated in axon guidance within the central nervous system through binding its high affinity receptor, plexin-B1 (3). Sema4D function has also been extensively characterized in the immune system and is the first semaphorin found to be expressed on the surface of many types of immune cells (4-6). In the immune system, CD72 serves as a low-affinity receptor for Sema4D (7) and research studies have shown that Sema4D not only regulates T-cell activation (8,9) but is also involved in the regulation of B-cell survival and differentiation (10). Many of the physiologic effects of Sema4D in the immune system are regulated by a soluble extracellular domain-containing fragment generated through proteolytic cleavage (11).

Sema4D has also been implicated in oncogenesis as research studies have demonstrated overexpression in multiple types of solid tumors (12,13). The role of Sema4D in oncogenesis, in part, has been linked to its ability to promote tumor angiogenesis (14), cell invasion (15), and immunosuppression through enhancement of myeloid derived suppressor cell function (16).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
5127   Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)
53108   Semaphorin-4D/CD100 (E5C3B) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8457   β-Actin (D6A8) Rabbit mAb
9863   Protein A Agarose Beads
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Semaphorin-4D/CD100 (E8S8A) Rabbit mAb

Immune Cell Signaling Pathways