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#8335 Fatty Acid and Lipid Metabolism Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#8335T1 Kit107,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
AceCS1 (D19C6) Rabbit mAb #3658 20 µl WB, IP H, M, R, Mk 78 Rabbit IgG
Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818 20 µl WB, IP, IHC-P, IF-IC H, M, R 280 Rabbit IgG
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 20 µl WB, IP, IHC-P, IF-IC, F H, M, R, Hm 280 Rabbit IgG
ATP-Citrate Lyase Antibody #4332 20 µl WB H, M, R, Mk 125 Rabbit
Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331 20 µl WB, IP H, M R 125 Rabbit
Fatty Acid Synthase (C20G5) Rabbit mAb #3180 20 µl WB, IP, IHC-P, IHC-F, IF-IC H, M, R B 273 Rabbit IgG
Lipin 1 (D2W9G) Rabbit mAb #14906 20 µl WB, IP, IF-IC H, M 130 Rabbit IgG
ACSL1 (D2H5) Rabbit mAb #9189 20 µl WB, IP H, M, R, Mk 78 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using ATP-Citrate Lyase Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated for the indicated times, using Phospho-ATP-Citrate Lyase (Ser455) Antibody (upper) or ATP-Citrate Lyase Antibody #4332 (lower).


Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Fatty Acid Synthase (C20G5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using AceCS1 (D19C6) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ACSL1 (D2H5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lipin 1 (D2W9G) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded breast carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 3T3-L1 adipocytes (differentiated, 7 days) using Lipin 1 (D2W9G) Rabbit mAb (red). Lipid droplets were labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IP

IP

Immunoprecipitation of Acetyl-CoA Carboxylase from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. Western blot was performed using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse liver using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lymphoma, showing staining of adipocytes, using Fatty Acid Synthase (C20G5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brown fat using Fatty Acid Synthase (C20G5) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen SKOV-3 xenograft using Fatty Acid Synthase (C20G5) Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Fatty Acid Synthase (C20G5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).


バックグラウンド

The processes of fatty acid and lipid metabolism are vital for cellular nutrient and energy maintenance. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1,2). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (4). ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for glucogenesis (5). Phosphorylation of ACL at Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (6). Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA (7). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (8-10). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in the liver and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (11).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
BODIPY is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Fatty Acid and Lipid Metabolism Antibody Sampler Kit

Metabolic Reprogramming in Disease

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