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#8337 Glycolysis Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#8337T1 Kit107,000
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Glycolysis 製品一覧

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
PKM2 (D78A4) XP® Rabbit mAb #4053 20 µl WB, IP, IHC-P, IF-IC, F H, M, R, Mk 60 Rabbit IgG
GAPDH (D16H11) XP® Rabbit mAb #5174 20 µl WB, IHC-P, IF-IC H, M, R, Mk Pg 37 Rabbit IgG
Pyruvate Dehydrogenase (C54G1) Rabbit mAb #3205 20 µl WB, IHC-P H, M, R, Mk 43 Rabbit IgG
Hexokinase I (C35C4) Rabbit mAb #2024 20 µl WB, IP, IHC-P, IF-IC H, M 102 Rabbit IgG
Hexokinase II (C64G5) Rabbit mAb #2867 20 µl WB H, M, R, Mk 102 Rabbit
LDHA (C4B5) Rabbit mAb #3582 20 µl WB, IHC-P, IF-IC H, Mk 37 Rabbit IgG
PKM1/2 (C103A3) Rabbit mAb #3190 20 µl WB, IF-IC H, M, R, Mk 60 Rabbit IgG
PFKP (D4B2) Rabbit mAb #8164 20 µl WB, IP H, Mk 80 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PKM1/2 (C103A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using LDHA (C4B5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and mouse skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PFKP (D4B2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of various cell extracts using Hexokinase II (C64G5) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using PKM1/2 (C103A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase I (C35C4) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LDHA (C4B5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PKM2 (D78A4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase I (C35C4) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using LDHA (C4B5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using PKM2 (D78A4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Hexokinase I (C35C4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb. Note the lack of staining in the skeletal muscle cells which do not express PKM2 while vessels within the tissue stain positively.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293T cells, transfected with PKM2 siRNA (blue) or mock transfected (green), using PKM2 (D78A4) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A204 cells using PKM2 (D78A4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Glycolysis Antibody Sampler Kit

Metabolic Reprogramming in Disease

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製品特集

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