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#8348 Rig-I Pathway Antibody Sampler Kit

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2019年3月20日11時40分 現在
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#8348T1 Kit115,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
MDA-5 (D74E4) Rabbit mAb #5321 20 µl WB, IP H, M 135 Rabbit IgG
Rig-I (D14G6) Rabbit mAb #3743 20 µl WB, IP H, M, R, Mk 102 Rabbit IgG
MAVS Antibody #3993 20 µl WB, IF-IC H 75, 52 Rabbit
IRF-3 (D6I4C) XP® Rabbit mAb #11904 20 µl WB, IP, IF-IC H, Mk 50-55 Rabbit IgG
TBK1/NAK (D1B4) Rabbit mAb #3504 20 µl WB, IP H, M, R, Mk 84 Rabbit
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb #5483 20 µl WB, IP, IF-IC, F H, M R, Mk, X, B, Dg 84 Rabbit IgG
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 20 µl WB H, M Mk, Pg 45-55 Rabbit IgG
Phospho-IKKε (Ser172) (D1B7) Rabbit mAb #8766 20 µl WB, IP H M, R, Mk, Dg 80 Rabbit IgG
IKKε (D20G4) Rabbit mAb #2905 20 µl WB, IP H Mk 80 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP, MCF-7, and HT29 cell lines using MAVS Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 and THP1 cells, control or plpC-transfected (1 hour), using Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of recombinant GST-IKKε #7553 and lysates from Ramos and RL7 cells using IKKε (D20G4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TBK1/NAK (D1B4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of differentiated THP-1 and RAW 264.7 cells, untreated or LPS-treated (1 ug/ml, overnight), using Rig-I (D14G6) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human MDA-5 (+), using MDA-5 (D74E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, indicated times), using Phospho-IKKε (Ser172) (D1B7) Rabbit mAb (upper) or IKKε (D20G4) Rabbit mAb #2905 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IRF-3 (D6I4C) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock-transfected or transfected with human MAVS, using MAVS Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, 1 hour), with or without phosphatase treatment using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).


Western Blotting

Western Blotting

Western blot anlaysis of extracts from differentiated THP-1 cells, untreated or treated with LPS (1 μg/ml, indicated times), using MDA-5 (D74E4) Rabbit mAb.

IP

IP

Immunoprecipitation of IRF-3 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IRF-3 (D6I4C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IRF-3 (D6I4C) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using MAVS Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells differentiated with TPA #9905, untreated (blue) or LPS-treated (green), using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells, untransfected (left) or transfected with Poly(I:C) (2.5 μg/ml, 6 hr; right), using IRF-3 (D6I4C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA #4174 (80nM, overnight) (left), followed by treatment with LPS (1μg/ml, 1 hour) (center) or LPS with λ phosphatase treatment (right) using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Rig-I Pathway Antibody Sampler Kit

Immune Cell Signaling Pathways

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