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#8349 NuRD Complex Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#8349T1 Kit99,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
CHD3 Antibody #4241 20 µl WB, IP H, M, Mk 260 Rabbit
CHD4 (D8B12) Rabbit mAb #11912 20 µl WB, IP H, M, R, Mk B, Pg, GP, Hr 260 Rabbit IgG
HDAC1 (10E2) Mouse mAb #5356 20 µl WB, IP H, M, R, Mk 62 Mouse IgG1
HDAC2 (3F3) Mouse mAb #5113 20 µl WB, IP, IF-IC H, M, R, Mk 60 Mouse IgG1
MBD3 (N87) Antibody #14540 20 µl WB H, M, R, Mk 32, 34 Rabbit
MTA1 (D40D1) XP® Rabbit mAb #5647 20 µl WB, IHC-P H, M, R, Mk 78-82 Rabbit IgG
RBAP46 (V415) Antibody #6882 20 µl WB, IP, IF-IC H, M, R, Mk Pg, Hr 48 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and Jurkat cells using CHD3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and A-204 cells using MTA1 (D40D1) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBAP46 (V415) Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CHD4 (D8B12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MBD3 (N87) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using RBAP46 (V415) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse 4T1A mouse syngeneic tumor using MTA1 (D40D1) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of CHD4 from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD4 (D8B12) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD4 (D8B12) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MTA1 (D40D1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

バックグラウンド

The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11).

使用例
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9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

NuRD Complex Antibody Sampler Kit

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